Detection of Viruses in Human Adenoid Tissues by Use of Multiplex PCR

被引：24

作者：

Sato, Masatoki ^{[1
,5
,6
]}

Li, Haijing ^{[2
]}

Ikizler, Mine R. ^{[1
]}

Werkhaven, Jay A. ^{[3
]}

Williams, John V. ^{[1
]}

Chappell, James D. ^{[1
,4
]}

Tang, Yi-Wei ^{[2
,4
]}

Wright, Peter F. ^{[1
,4
,6
]}

机构：

[1] Vanderbilt Univ, Sch Med, Dept Pediat, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA

[3] Vanderbilt Univ, Sch Med, Dept Otolaryngol, Nashville, TN 37232 USA

[4] Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA

[5] Fukushima Med Univ, Sch Med, Dept Pediat, Fukushima 9601295, Japan

[6] Dartmouth Med Sch, Dept Pediat, Hanover, NH 03755 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 03期

关键词：

POLYMERASE-CHAIN-REACTION; HUMAN HERPESVIRUS-7; BETA-HERPESVIRUSES; WALDEYERS RING; T-CELL; SALIVA; DNA; LYMPHOCYTES; RHINOVIRUS; INFECTION;

D O I：

10.1128/JCM.02331-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By PCR, we detected a high frequency of viruses in adenoids obtained from children without acute respiratory symptoms. Our results suggest that persistent/latent viral infection in the respiratory tract confounds interpretation of the association of pathogen detection by PCR with acute respiratory infection in these sources.

引用

页码：771 / 773

页数：3

共 23 条

[1] Distribution patterns of β- and γ-herpesviruses within Waldeyer's ring organs [J].

Berger, Christoph ;

Hug, Martina ;

Gysin, Claudine ;

Molinari, Luciano ;

Frei, Martina ;

Bossart, Walter ;

Nadal, David .

JOURNAL OF MEDICAL VIROLOGY, 2007, 79 (08) :1147-1152

[2]

BROOK I, 1987, OTOLARYNG CLIN N AM, V20, P259

[3] DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN HUMAN TONSILLAR LYMPHOCYTES [J].

DAVID, D ;

RAVID, Z ;

MORAG, A .

JOURNAL OF MEDICAL VIROLOGY, 1987, 23 (04) :383-391

[4] Detection of respiratory viruses and atypical bacteria in children's tonsils and adenoids [J].

Drago, Lorenzo ;

Esposito, Susanna ;

De Vecchi, Elena ;

Marchisio, Paola ;

Blasi, Francesco ;

Baggi, Elena ;

Capaccio, Pasquale ;

Pignatar, Lorenzo .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) :369-370

[5] Detection of Epstein-Barr virus in tonsillar tissue of children and the relationship with recurrent tonsillitis [J].

Endo, LH ;

Ferreira, D ;

Montenegro, MCS ;

Pinto, GA ;

Altemani, A ;

Bortoleto, AE ;

Vassallo, J .

INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY, 2001, 58 (01) :9-15

[6] A previously undescribed coronavirus associated with respiratory disease in humans [J].

Fouchier, RAM ;

Hartwig, NG ;

Bestebroer, TM ;

Niemeyer, B ;

de Jong, JC ;

Simon, JH ;

Osterhaus, ADME .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (16) :6212-6216

[7] Prevalence and quantitation of species C adenovirus DNA in human mucosal lymphocytes [J].

Garnett, CT ;

Erdman, D ;

Xu, W ;

Gooding, LR .

JOURNAL OF VIROLOGY, 2002, 76 (21) :10608-10616

[8] FREQUENT ISOLATION OF HUMAN HERPESVIRUS-7 FROM SALIVA SAMPLES [J].

HIDAKA, Y ;

LIU, Y ;

YAMAMOTO, M ;

MORI, R ;

MIYAZAKI, C ;

KUSUHARA, K ;

OKADA, K ;

UEDA, K .

JOURNAL OF MEDICAL VIROLOGY, 1993, 40 (04) :343-346

[9] Variation of human herpesvirus 7 shedding in saliva [J].

Ihira, M ;

Yoshikawa, T ;

Ohashi, M ;

Enomono, Y ;

Akimoto, S ;

Suga, S ;

Saji, H ;

Nishiyama, Y ;

Asano, Y .

JOURNAL OF INFECTIOUS DISEASES, 2003, 188 (09) :1352-1354

[10] Detection of reovirus by reverse transcription-polymerase chain reaction using primers corresponding to conserved regions of the viral L1 genome segment [J].

Leary, TP ;

Erker, JC ;

Chalmers, ML ;

Wetzel, JD ;

Desai, SM ;

Mushahwar, IK ;

Dermody, TS .

JOURNAL OF VIROLOGICAL METHODS, 2002, 104 (02) :161-165

← 1 2 3 →