Structure of the Michaelis complex of β-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis

被引：19

作者：

Offen, Wendy A. ^{[1
]}

Zechel, David L. ^{[2
]}

Withers, Stephen G. ^{[3
]}

Gilbert, Harry J. ^{[4
]}

Davies, Gideon J. ^{[1
]}

机构：

[1] Univ York, Dept Chem, York Struct Biol Lab, York YO10 5YW, N Yorkshire, England

[2] Queens Univ, Dept Chem, Kingston, ON K7L 3N6, Canada

[3] Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z3, Canada

[4] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA

来源：

CHEMICAL COMMUNICATIONS | 2009年 / 18期

基金：

英国生物技术与生命科学研究理事会; 加拿大自然科学与工程研究理事会;

关键词：

SELECTIVE-INHIBITION; MECHANISM; GLYCOSIDASES; MANNOSTATIN;

D O I：

10.1039/b902240f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The Michaelis complex of the beta-mannosidase Man2A shows distortion to a S-1(5) conformation adding to the growing body of evidence supporting catalysis through a boat conformation.

引用

页码：2484 / 2486

页数：3

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