Architectural requirements for optimal activation by tandem CRP molecules at a class ICRP-dependent promoter

被引：25

作者：

Tebbutt, J ^{[1
]}

Rhodius, VA ^{[1
]}

Webster, CL ^{[1
]}

Busby, SJW ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 210卷 / 01期

关键词：

transcription activation; cAMP receptor protein; RNA polymerase alpha subunit; CRP-dependent promoter;

D O I：

10.1111/j.1574-6968.2002.tb11159.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：55 / 60

页数：6

共 10 条

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