Separation behavior of avidin and related substances using a combination of zwitterionic surfactant and C8 phase capillary

被引：6

作者：

Mori, M ^{[1
]}

Kodama, K ^{[1
]}

Hu, WZ ^{[1
]}

Tanaka, S ^{[1
]}

机构：

[1] Hokkaido Univ, Div Sci Mat, Grad Sch Environm Earth Sci, Sapporo, Hokkaido 0600810, Japan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1999年 / 22卷 / 20期

关键词：

D O I：

10.1081/JLC-100102081

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of mixtures of avidin, streptavidin, and neutravidin were investigated by using combinations of C-8 phase capillary and some surfactants such as anionic, cationic, zwitterionic, nonionic surfactants, polyvinyl alcohol (PVA), and hydroxyl cellulose (HEC) polymers. The combination of zwittergent 3 - 14 and C-8 phase capillary gave the best separation of avidin and its relatives. The satisfactory reproducibility of the peak height and the migration time were achieved by this system. The complete separation of the mixture of avidin, streptavidin, neutravidin, and other three basic proteins was achieved with sharp peaks in pH 5, 7 and 9 of running buffers.

引用

页码：3139 / 3150

页数：12

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