T cell CD3 receptor zeta (TCRζ)-chain expression in children with idiopathic nephrotic syndrome

被引:7
作者
Aviles, Diego H. [1 ]
Vehaskari, Matti V. [1 ]
Culotta, Kirk S. [2 ]
Manning, Jennifer [1 ]
Ochoa, Augusto C. [1 ,3 ]
Zea, Arnold H. [4 ]
机构
[1] LSU Hlth Sci Ctr, Dept Pediat, New Orleans, LA 70112 USA
[2] Univ Texas MD Anderson Canc Ctr, Pharmacol Res Div Pharm, Houston, TX 77030 USA
[3] Stanley C Scott Canc Ctr, Tumor Immunol Program, New Orleans, LA USA
[4] Stanley S Scott Canc Ctr, New Orleans, LA USA
基金
美国国家卫生研究院;
关键词
Lymphocyte; Glomerular disease; Nephrotic syndrome; T-cell receptor; Zeta chain; DECREASED EXPRESSION; SIGNAL-TRANSDUCTION; CHAIN; LYMPHOCYTES; CHILDHOOD; PROTEIN; SERUM; ARGININE; CD3-ZETA; DISEASE;
D O I
10.1007/s00467-008-1061-1
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Children with idiopathic nephrotic syndrome (INS) have an increased risk of developing life-threatening infections. Several studies have demonstrated functional abnormalities in the T lymphocytes of patients with nephrotic syndrome. Although T cells are activated in INS during relapse, as indicated by an increased expression of interleukin (IL)-2 receptor, these cells have a decreased ability to proliferate. The T-cell receptor (TCR) plays an important role in signal transduction and T cell activation, with the TCR-zeta (TCR zeta) chain being a key element in early signaling. We measured the expression of the TCR zeta chain in patients with INS (steroid resistant and steroid sensitive) during relapse and remission by flow cytometry and by PCR ELISA. The results showed a significant decrease in the expression of the TCR zeta chain at both the protein and mRNA level in INS patients during relapse as compared with normal controls (p < 0.05). In contrast, when patients with INS achieved remission, the expression of TCR zeta normalized and was similar to that expressed in normal controls. Therefore, a decreased expression of the TCR zeta chain may explain the abnormal function of T cells in patients with INS, and it may also contribute to the increased risk for infections seen in these patients.
引用
收藏
页码:769 / 773
页数:5
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