Leaf wounding increases efficiency of Agrobacterium-mediated transformation of apple

被引：61

作者：

Norelli, J

Mills, J

Aldwinckle, H

机构：

来源：

HORTSCIENCE | 1996年 / 31卷 / 06期

关键词：

Agrobacterium tumefaciens; Malus xdomestica;

D O I：

10.21273/HORTSCI.31.6.1026

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

In vitro-grown leaves of Malus xdomestica Borkh. cv. Royal Gala were either crush-wounded with forceps, cut, or left whole, and then inoculated with A. tumefaciens strain EHA105 (p35SGUS-INT), with and without vacuum infiltration. Transformation was quantified 13 days after inoculation by determining the rate of beta-glucuronidase (GUS) activity, Leaf wounding by crushing with nontraunatic forceps significantly increased transformation when compared with cutting leaves. Vacuum infiltration of inoculum had no effect on transformation.

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收藏

页码：1026 / 1027

页数：2

相关论文

共 13 条

[1]

BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194

[2] AN ANALYSIS OF TRANSFORMATIONS [J].

BOX, GEP ;

COX, DR .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1964, 26 (02) :211-252

[3] AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE (MALUS X DOMESTICA BORKH) - AN ASSESSMENT OF FACTORS AFFECTING GENE-TRANSFER EFFICIENCY DURING EARLY TRANSFORMATION STEPS [J].

DEBONDT, A ;

EGGERMONT, K ;

DRUART, P ;

DEVIL, M ;

GODERIS, I ;

VANDERLEYDEN, J ;

BROEKAERT, WE .

PLANT CELL REPORTS, 1994, 13 (10) :587-593

[4] ACETOSYRINGONE AND OSMOPROTECTANTS LIKE BETAINE OR PROLINE SYNERGISTICALLY ENHANCE AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE [J].

JAMES, DJ ;

URATSU, S ;

CHENG, JS ;

NEGRI, P ;

VISS, P ;

DANDEKAR, AM .

PLANT CELL REPORTS, 1993, 12 (10) :559-563

[5]

JAMES DJ, 1989, PLANT CELL REP, V7, P658, DOI 10.1007/BF00272054

[6]

JEFFERSON RA, 1987, EMBO J, V6, P3901

[7]

LIU ZR, 1987, THESIS CORNELL U ITH

[8] TRANSFORMATION OF APPLE ROOTSTOCK M26 WITH AGROBACTERIUM-TUMEFACIENS [J].

MAHESWARAN, G ;

WELANDER, M ;

HUTCHINSON, JF ;

GRAHAM, MW ;

RICHARDS, D .

JOURNAL OF PLANT PHYSIOLOGY, 1992, 139 (05) :560-568

[9] THE ROLE OF AMINOGLYCOSIDE ANTIBIOTICS IN THE REGENERATION AND SELECTION OF NEOMYCIN PHOSPHOTRANSFERASE-TRANSGENIC APPLE TISSUE [J].

NORELLI, JL ;

ALDWINCKLE, HS .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1993, 118 (02) :311-316

[10]

Sambrook J., 2002, MOL CLONING LAB MANU