Coupling of electrochemistry and fluorescence microscopy at indium tin oxide microelectrodes for the analysis of single exocytotic events

被引:74
作者
Amatore, C [1 ]
Arbault, S [1 ]
Chen, Y [1 ]
Crozatier, C [1 ]
Lemaître, F [1 ]
Verchier, Y [1 ]
机构
[1] Ecole Normale Super, UMR 8640, CNRS, UPMC PASTEUR,Dept Chim, F-75231 Paris 05, France
关键词
analytical methods; electrochemistry; exocytosis; fluorescence; indium-tin oxide;
D O I
10.1002/anie.200600510
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
(Figure Presented) One-off analysis: Quantitative, kinetic, and spatial monitoring of single biological events involving vesicular secretion such as exocytosis may be achieved by combining electrochemistry with fluorescence microscopy at indium tin oxide (ITO) transparent microelectrodes. This method paves the way for real-time tracking of the intracellular fate of vesicles while monitoring precisely their dynamics of fusion. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:4000 / 4003
页数:4
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