Coupling of electrochemistry and fluorescence microscopy at indium tin oxide microelectrodes for the analysis of single exocytotic events

被引：74

作者：

Amatore, C ^{[1
]}

Arbault, S ^{[1
]}

Chen, Y ^{[1
]}

Crozatier, C ^{[1
]}

Lemaître, F ^{[1
]}

Verchier, Y ^{[1
]}

机构：

[1] Ecole Normale Super, UMR 8640, CNRS, UPMC PASTEUR,Dept Chim, F-75231 Paris 05, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 24期

关键词：

analytical methods; electrochemistry; exocytosis; fluorescence; indium-tin oxide;

D O I：

10.1002/anie.200600510

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) One-off analysis: Quantitative, kinetic, and spatial monitoring of single biological events involving vesicular secretion such as exocytosis may be achieved by combining electrochemistry with fluorescence microscopy at indium tin oxide (ITO) transparent microelectrodes. This method paves the way for real-time tracking of the intracellular fate of vesicles while monitoring precisely their dynamics of fusion. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4000 / 4003

页数：4

共 18 条

[1] Correlation between vesicle quantal size and fusion pore release in chromaffin cell exocytosis [J].

Amatore, C ;

Arbault, S ;

Bonifas, I ;

Bouret, Y ;

Erard, M ;

Ewing, AG ;

Sombers, LA .

BIOPHYSICAL JOURNAL, 2005, 88 (06) :4411-4420

[2]

Amatore C, 2000, ANGEW CHEM INT EDIT, V39, P1952, DOI 10.1002/1521-3773(20000602)39:11<1952::AID-ANIE1952>3.0.CO

[3]

2-3

[4] Dynamics of full fusion during vesicular exocytotic events: Release of adrenaline by chromaffin cells [J].

Amatore, C ;

Arbault, S ;

Bonifas, I ;

Bouret, Y ;

Erard, M ;

Guille, M .

CHEMPHYSCHEM, 2003, 4 (02) :147-154

[5]

[Anonymous], 2000, ANGEW CHEM

[6] Secretory granule exocytosis [J].

Burgoyne, RD ;

Morgan, A .

PHYSIOLOGICAL REVIEWS, 2003, 83 (02) :581-632

[7] Patch amperometry: high-resolution measurements of single-vesicle fusion and release [J].

Dernick, G ;

Gong, LW ;

Tabares, L ;

de Toledo, GA ;

Lindau, M .

NATURE METHODS, 2005, 2 (09) :699-708

[8] RELEASE OF SECRETORY PRODUCTS DURING TRANSIENT VESICLE FUSION [J].

DETOLEDO, GA ;

FERNANDEZCHACON, R ;

FERNANDEZ, JM .

NATURE, 1993, 363 (6429) :554-558

[9] The fusion pore [J].

Lindau, M ;

de Toledo, GA .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2003, 1641 (2-3) :167-173

[10] ADRENAL-MEDULLARY CHROMAFFIN CELLS-INVITRO [J].

LIVETT, BG .

PHYSIOLOGICAL REVIEWS, 1984, 64 (04) :1103-1161

← 1 2 →