Kinetic study of direct bioelectrocatalysis of dioxygen reduction with bilirubin oxidase at carbon electrodes

被引：133

作者：

Tsujimura, S ^{[1
]}

Nakagawa, T ^{[1
]}

Kano, K ^{[1
]}

Ikeda, T ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto 6068502, Japan

来源：

ELECTROCHEMISTRY | 2004年 / 72卷 / 06期

关键词：

biofuel cell; direct electron transfer; bilirubin oxidase; carbon electrode;

D O I：

10.5796/electrochemistry.72.437

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Direct electron transfer-type bioelectrocatalysis of four-electron reduction of dioxygen was successfully realized with bilirubin oxidase (BOD, EC 1.3.3.5, from Myrothecium verrucaria) at carbon electrode surfaces with high crystal graphite edge density. It was found that adsorbed BOD functions as a biocatalyst. The current-potential curves were interpreted by considering the enzyme catalytic constant, surface electron transfer kinetics, surface concentration of BOD, and the formal potential of BOD. The analysis suggested that the standard surface electron transfer rate constant and surface coverage of "active" enzyme depend strongly on functional groups and/or nano-structure of carbon electrode surface.

引用

页码：437 / 439

页数：3

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