IgE-binding and cross-reactivity of a new 41 kDa allergen of codfish

被引:37
作者
Das Dores, S
Chopin, C
Romano, A
Galland-Irmouli, AV
Quaratino, D
Pascual, C
Fleurence, J
Guéant, JL
机构
[1] Univ Henri Poincare, Fac Med Nancy, IFREMER,URM 20,INSERM,EMI 0014, Lab Pathol Cellulaire & Mol Nutr, F-54505 Vandoeuvre Les Nancy, France
[2] IFREMER, Lab Prot Qual, Nantes, France
[3] CI Columbus, UCSC Allergy Unit, Dept Internal Med & Geriatr, Rome, Italy
[4] IRCCS Oasi Maria SS, Troina, Italy
[5] Hosp Infantil La Paz, Lab Immunoalergia, Madrid, Spain
关键词
codfish; dehydrogenase; food allergy; IgE;
D O I
10.1034/j.1398-9995.57.s72.6.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: A 41-kDa IgE-reactive protein (p41) was purified from raw cod extract. This protein is homologous to an aldehyde phosphate dehydrogenase (APDH). The present study aims to evaluate the IgE-binding and the cross-reactivity of this protein in 13 patients allergic to codfish. Methods: IgE binding of sera from 13 patients allergic to codfish was tested by Sepharose RIA and by Western blot. Results: Among the 13 patients, only 4 had specific IgE to APDH detected by APDH-Sepharose RIA. The two patients who had the highest level of specific IgE to human APDH also had a class 5-6 CAP-RAST IgE level to codfish, but two other patients with a class 5 had a negative APDH-Sepharose IgE-RIA. Relative content of APDH was higher in extracts of commercial nonfrozen fish, compared to pre rigor mortis, post rigor mortis and frozen commercial codfish. A high homology of codfish APDH was found with the corresponding human enzyme. A significant inhibition of APDH-Sepharose by human and, to a lesser extent, by rabbit APDH was observed. Western blot of APDH codfish extract showed two bands at 41 and 36 kDa, respectively. Conclusions: We have characterized a new allergen from codfish, which had a high level of homology in different species. The p41 relative content of extracts from nonfrozen codfish was higher than in the other samples assessed.
引用
收藏
页码:84 / 87
页数:4
相关论文
共 23 条
[1]   STUDIES OF HYPERSENSITIVITY TO FISH - A CLINICAL STUDY [J].
AAS, K .
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1966, 29 (04) :346-&
[2]   CROSS-ALLERGENICITY IN THE LEGUME BOTANICAL FAMILY IN CHILDREN WITH FOOD HYPERSENSITIVITY .2. LABORATORY CORRELATES [J].
BERNHISELBROADBENT, J ;
TAYLOR, S ;
SAMPSON, HA .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1989, 84 (05) :701-709
[3]   FISH HYPERSENSITIVITY .1. INVITRO AND ORAL CHALLENGE RESULTS IN FISH-ALLERGIC PATIENTS [J].
BERNHISELBROADBENT, J ;
SCANLON, SM ;
SAMPSON, HA .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 89 (03) :730-737
[4]   Adverse reactions and intolerance to foods [J].
David, TJ .
BRITISH MEDICAL BULLETIN, 2000, 56 (01) :34-50
[5]  
DEMARTINO M, 1993, ANN ALLERGY, V71, P159
[6]   ALLERGY TO DIFFERENT FISH SPECIES IN COD-ALLERGIC CHILDREN - INVIVO AND INVITRO STUDIES [J].
DEMARTINO, M ;
NOVEMBRE, E ;
GALLI, L ;
DEMARCO, A ;
BOTARELLI, P ;
MARANO, E ;
VIERUCCI, A .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1990, 86 (06) :909-914
[7]   Recognition of an extensive range of IgE-reactive proteins in cod extract [J].
Dory, D ;
Chopin, C ;
Aimone-Gastin, I ;
Gueant, JL ;
Guerin, L ;
Sainte-Laudy, J ;
Moneret-Vautrin, DA ;
Fleurence, J .
ALLERGY, 1998, 53 (01) :42-50
[8]   Purification of a 41 kDa cod-allergenic protein [J].
Galland, AV ;
Dory, D ;
Pons, L ;
Chopin, C ;
Rabesona, H ;
Guéant, JL ;
Fleurence, J .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 706 (01) :63-71
[9]  
GUEANT JL, 1991, ALLERGY, V46, P452
[10]  
GUEANT JL, 1992, MONOGR ALLERGY, V30, P94