Development of a novel rhodamine-type fluorescent probe to determine peroxynitrite

被引：267

作者：

Yang, XF

Guo, XQ ^{[1
]}

Zhao, YB

机构：

[1] Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China

[2] Xiamen Univ, Key Lab Analyt Sci MOE, Xiamen 361005, Peoples R China

来源：

TALANTA | 2002年 / 57卷 / 05期

关键词：

peroxynitrite; rhodamine B hydrazide; fluorimetry; probe;

D O I：

10.1016/S0039-9140(02)00120-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method for the determination of peroxynitrite using rhodamine B hydrazide as a fluorogenic probe is described. The method is based on the oxidation of rhodamine B hydrazide, a colorless, non-fluorescent substance, by peroxynitrite to give rhodamine B-like fluorescence emission. The fluorescence increase is linearly related to the concentration of peroxynitrite in the range of 7.5 x 10(-8)-3.0 x 10(-6) mol l(-1) with a detection limit of 2.4 x 10(-8) mol l(-1) (3sigma). The optimal conditions for the detection of peroxynitrite were evaluated and the possible detection mechanism was also discussed in this paper. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：883 / 890

页数：8

相关论文

共 23 条

[1] Hydroxyl radical formation during peroxynitrous acid decomposition [J].

Coddington, JW ;

Hurst, JK ;

Lymar, SV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (11) :2438-2443

[2] Peroxynitrite irreversibly decreases diastolic and systolic function in cardiac muscle [J].

Digerness, SB ;

Harris, KD ;

Kirklin, JW ;

Urthaler, F ;

Viera, L ;

Beckman, JS ;

Darley-Usmar, V .

FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (11-12) :1386-1392

[3] A long-wavelength fluorescent chemodosimeter selective for Cu(II) ion in water [J].

Dujols, V ;

Ford, F ;

Czarnik, AW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (31) :7386-7387

[4] Clinical evidence of peroxynitrite formation in chronic renal failure patients with septic shock [J].

Fukuyama, N ;

Takebayashi, Y ;

Hida, M ;

Ishida, H ;

Ichimori, K ;

Nakazawa, H .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :771-774

[5] THE REACTION OF NO-CENTER-DOT WITH O-2(CENTER-DOT-) AND HO2-CENTER-DOT - A PULSE-RADIOLYSIS STUDY [J].

GOLDSTEIN, S ;

CZAPSKI, G .

FREE RADICAL BIOLOGY AND MEDICINE, 1995, 19 (04) :505-510

[6] DIRECT AND INDIRECT OXIDATIONS BY PEROXYNITRITE [J].

GOLDSTEIN, S ;

CZAPSKI, G .

INORGANIC CHEMISTRY, 1995, 34 (16) :4041-4048

[7] Dihydrofluorescein diacetate is superior for detecting intracellular oxidants:: Comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123 [J].

Hempel, SL ;

Buettner, GR ;

O'Malley, YQ ;

Wessels, DA ;

Flaherty, DM .

FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (1-2) :146-159

[8] CHEMISTRY OF PERNITRITES .I. KINETICS OF DECOMPOSITION OF PERNITROUS ACID [J].

HUGHES, MN ;

NICKLIN, HG .

JOURNAL OF THE CHEMICAL SOCIETY A -INORGANIC PHYSICAL THEORETICAL, 1968, (02) :450-&

[9] AUTOXIDATION OF HYDROXYLAMINE IN ALKALINE SOLUTIONS [J].

HUGHES, MN ;

NICKLIN, HG .

JOURNAL OF THE CHEMICAL SOCIETY A -INORGANIC PHYSICAL THEORETICAL, 1971, (01) :164-&

[10] Oxidation of 2',7'-dichlorofluorescin by peroxynitrite [J].

Kooy, NW ;

Royall, JA ;

Ischiropoulos, H .

FREE RADICAL RESEARCH, 1997, 27 (03) :245-254