Overexpressing both ATP sulfurylase and selenocysteine methyltransferase enhances selenium phytoremediation traits in Indian mustard

被引:102
作者
Leduc, Danika L.
AbdelSamie, Manal
Montes-Bayon, Maria
Wu, Carol P.
Reisinger, Sarah J.
Terry, Norman [1 ]
机构
[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[2] Univ Oviedo, Dept Quim Fis & Analyt, Asturias 33006, Spain
基金
美国国家科学基金会;
关键词
selenium; Indian mustard; selenocysteine methyltransferase; ATP sulfurylase;
D O I
10.1016/j.envpol.2006.01.008
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A major goal of our selenium (Se) phytoremediation research is to use genetic engineering to develop fast-growing plants with an increased ability to tolerate, accumulate, and volatilize Se. To this end we incorporated a gene (encoding selenocysteine methyltransferase, SMT) from the Se hyperaccumulator, Astragalus bisulcatus, into Indian mustard (LeDuc, D.L., Tarun, A.S., Montes-Bayon, M., Meija, J., Malit, M.F, Wu, C.P., AbdelSamie, M., Chiang, C.-Y., Tagmount, A., deSouza, M., Neuhierl, B., Bock, A., Caruso, J., Terry, N., 2004. Overexpression of selenocysteine methyltransferase in Arabidopsis and Indian mustard increases selenium tolerance and accumulation Plant Physiol. 135, 377-383.). The resulting transgenic plants successfully enhanced Se phytoremediation in that the plants tolerated and accumulated Se from selenite significantly better than wild type. However, the advantage confer-red by the SMT enzyme was much less when Se was supplied as selenate. In order to enhance the phytoremediation of selenate, we developed double transgenic plants that overexpressed the gene encoding ATP sulfurylase (APS) in addition to SMT, i.e., APS x SMT. The results showed that there was a substantial improvement in Se accumulation from selenate (4 to 9 times increase) in transgenic plants overexpressing both APS and SMT. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:70 / 76
页数:7
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