Differential expression of two fructokinases in Oryza sativa seedlings grown under aerobic and anaerobic conditions

被引:33
作者
Guglielminetti, L.
Morita, A.
Yamaguchi, J.
Loreti, E.
Perata, P.
Alpi, A.
机构
[1] Dipartimento Biol Piante Agr, Sez Fisiol Vegetale, I-56124 Pisa, Italy
[2] Nagoya Univ, Biosci Ctr, Nagoya, Aichi, Japan
[3] Hokkaido Univ, Fac Sci, Dept Biol Sci, Sapporo, Hokkaido 060, Japan
[4] CNR, Sez Pisa, Ist Biol & Biotecnol Agraria, I-56100 Pisa, Italy
[5] Univ Pisa, Scuola Super Sant Anna Studi, I-56100 Pisa, Italy
[6] Perfezionamento, Pisa, Italy
基金
日本学术振兴会;
关键词
anoxia; carbohydrate; fructokinase; seedling; rice;
D O I
10.1007/s10265-006-0281-3
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
Fructokinases (EC 2.7.1.4) may play an important role in carbohydrate metabolism of Oryza sativa L. (rice) seedlings under anoxia. We present here the molecular and biochemical characterizations of two rice fructokinases, namely OsFK1 and OsFK2. The results show that, at both a transcriptional and a transductional level, OsFK1 is preferentially expressed under aerobic conditions, whereas OsFK2 is induced under anoxia. Substrate inhibition was demonstrated for OsFK1, while OsFK2 appears to be largely unaffected by fructose concentrations up to 10 mM. Sugar modulation of anoxia-induced proteins has been proposed, but our results on rice calli treated with or without glucose (10, 30 or 90 mM) for different time indicate that neither OsFK1 nor OsFK2 are sugar-regulated. We propose that OsFK2 plays a major role in fructose phosphorylation under anoxic conditions.
引用
收藏
页码:351 / 356
页数:6
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