In vitro Derby Imaging of Cancer Biomarkers Using Quantum Dots

被引：62

作者：

Ko, Mee Hyang ^{[2
]}

Kim, Soonhag ^{[1
]}

Kang, Won Jun ^{[4
]}

Lee, Jung Hwan ^{[3
]}

Kang, Hyungu ^{[3
]}

Moon, Sung Hwan ^{[3
]}

Hwang, Do Won ^{[2
]}

Ko, Hae Young ^{[2
]}

Lee, Dong Soo ^{[2
]}

机构：

[1] Seoul Natl Univ, Coll Med, Med Res Ctr, Seoul 110744, South Korea

[2] Seoul Natl Univ, Coll Med, Dept Nucl Med, Seoul 110744, South Korea

[3] Pohang Univ Sci & Technol, Postech Biotech Ctr, Aptamer Unit, Pohang, Kyungbuk, South Korea

[4] Yonsei Univ, Coll Med, Dept Radiol, Div Nucl Med, Seoul, South Korea

来源：

SMALL | 2009年 / 5卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

derby imaging; nucleolin aptamers; peptides; quantum dots; INTEGRIN ALPHA-V-BETA-3; TUMOR-CELLS; EXTRACELLULAR SEGMENT; CRYSTAL-STRUCTURE; BINDING; VIVO; EXPRESSION; NUCLEOLIN; COMPLEX; PROTEIN;

D O I：

10.1002/smll.200801580

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Semiconductor quantum dots (QDs), which have broad absorption with narrow. emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and the arginine-glycine-aspartic acid (targeting the integrin alpha(v)beta(3)) in cancer cells is reported. Simultaneous fluorescence imaging of cellular distribution of nucleolin and integrin alpha(v)beta(3) using QDs enables easy monitoring of separate targets in the cancer cells and the normal healthy cells. These results suggest the feasibility of a concurrent visualization of QD-based multiple cancer biomarkers using small molecules such as aptamer or peptide ligands.

引用

页码：1207 / 1212

页数：6

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