A technique for the in vivo study of multiple stable isotope-labeled essential fatty acids

被引:6
作者
Lin, Y [1 ]
Salem, N [1 ]
机构
[1] NIAAA, Sect Nutr Neurosci, Lab Membrane Biochem & Biophys, NIH, Rockville, MD 20852 USA
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 2002年 / 67卷 / 2-3期
关键词
D O I
10.1054/plef.2002.0411
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel tracer technique is presented for the simultaneous and independent measurement of multiple stable isotopically labeled essential fatty acids. Gas chromatography/negative chemical ionization mass spectrometry was employed for high sensitivity detection of the following isotopes: deuterium-labeled-linolenate, carbon-13-U-labeled-eicosapentaenoate, carbon-13-U-labeled-linoleate, and deuterium-labeled-dihomo-gamma-linolenate. These isotope-labeled fatty acids in vehicle oil were given to rats either singly or together as a single oral dose. Rat blood was collected after dosing and the isotopomers of the precursors and their main metabolites, including those containing both C-13 and H-2, were detected simultaneously with good resolution and without interference from other isotopes due to differences in mass and chromatographic retention. Published by Elsevier Science Ltd.
引用
收藏
页码:141 / 146
页数:6
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