Disseminated Tumor Cells Persist in the Bone Marrow of Breast Cancer Patients through Sustained Activation of the Unfolded Protein Response

被引:74
作者
Bartkowiak, Kai [1 ]
Kwiatkowski, Marcel [2 ]
Buck, Friedrich [2 ]
Gorges, Tobias M. [1 ]
Nilse, Lars [3 ]
Assmann, Volker [1 ]
Andreas, Antje [1 ]
Mueller, Volkmar [4 ]
Wikman, Harriet [1 ]
Riethdorf, Sabine [1 ]
Schlueter, Hartmut [2 ]
Pantel, Klaus [1 ]
机构
[1] Univ Med Ctr Hamburg Eppendorf, Dept Tumor Biol, D-20246 Hamburg, Germany
[2] Univ Med Ctr Hamburg Eppendorf, Inst Clin Chem, D-20246 Hamburg, Germany
[3] Univ Freiburg, Inst Mol Med & Cell Res, D-79106 Freiburg, Germany
[4] Univ Med Ctr Hamburg Eppendorf, Dept Gynecol, D-20246 Hamburg, Germany
关键词
VASCULOGENIC MIMICRY; STEM-CELLS; HEMATOPOIETIC STEM; PROSTATE-CANCER; POOLED ANALYSIS; RNA GRANULES; HYPOXIA; MIGRATION; MICROMETASTASIS; SURVIVAL;
D O I
10.1158/0008-5472.CAN-14-3728
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
Disseminated tumor cells (DTC), which share mesenchymal and epithelial properties, are considered to be metastasis-initiating cells in breast cancer. However, the mechanisms supporting DTC survival are poorly understood. DTC extravasation into the bone marrow may be encouraged by low oxygen concentrations that trigger metabolic and molecular alterations contributing to DTC survival. Here, we investigated how the unfolded protein response (UPR), an important cytoprotective program induced by hypoxia, affects the behavior of stressed cancer cells. DTC cell lines established from the bone marrow of patients with breast cancer (BC-M1), lung cancer, (LC-M1), and prostate cancer (PC-E1) were subjected to hypoxic and hypoglycemic conditions. BC-M1 and LC-M1 exhibiting mesenchymal and epithelial properties adapted readily to hypoxia and glucose starvation. Upregulation of UPR proteins, such as the glucose-regulated protein Grp78, induced the formation of filamentous networks, resulting in proliferative advantages and sustained survival under total glucose deprivation. High Grp78 expression correlated with mesenchymal attributes of breast and lung cancer cells and with poor differentiation in clinical samples of primary breast and lung carcinomas. In DTCs isolated from bone marrow specimens from breast cancer patients, Grp78-positive stress granules were observed, consistent with the likelihood these cells were exposed to acute cell stress. Overall, our findings provide the first evidence that the UPR is activated in DTC in the bone marrow from cancer patients, warranting further study of this cell stress pathway as a predictive biomarker for recurrent metastatic disease. (C)2015 AACR. .
引用
收藏
页码:5367 / 5377
页数:11
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