ICAM-1-, -2- and LFA-1-independent homotypic T cell aggregation induced by a novel activating monoclonal antibody targeting the murine THY-1 molecule

被引:2
作者
Fan, XH [1 ]
Benz, P [1 ]
Wuthrich, RP [1 ]
机构
[1] UNIV ZURICH,DIV NEPHROL,ZURICH,SWITZERLAND
关键词
D O I
10.1007/BF01486742
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have previously reported that the murine T cell line EL-4 has an aggregating phenotype, displaying homotypic aggregation (HTA) when exposed to monoclonal antibodies targeting specific cell surface molecules such as leukocyte function-associated antigen-1 (LFA-1). We have used this property of EL-4 cells to isolate additional HTA-inducing MAb by screening a panel of hybridomas that were generated from rats immunized with EL-4 cells. We have isolated a novel anti-Thy-1 MAb (termed FF-10) that is a powerful inducer of HTA in EL-4 cells. In addition to induction of HTA, FF-10 also induces splenocyte proliferation but inhibits anti-CD3-driven T cell proliferation. Thy-1-induced HTA cannot be blocked with MAb targeting intercellular adhesion molecule-1 and -2 (ICAM-1, ICAM-2) or LFA-1. Thus, the FF-10 MAb represents a novel and unique tool to investigate the diverse roles of the murine Thy-1 molecule in T cell activation, proliferation and apoptosis.
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收藏
页码:401 / 411
页数:11
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