Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(D,L-lactic-co-glycolic acid) meshes

被引:320
作者
Bashur, Chris A.
Dahlgren, Linda A.
Goldstein, Aaron S. [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Dept Chem Engn, Blacksburg, VA 24061 USA
[2] Virginia Polytech Inst & State Univ, Sch Biomed Engn & Sci, Blacksburg, VA 24061 USA
[3] Virginia Maryland Reg Coll Vet Med, Dept Large Anim Clin Sci, Blacksburg, VA 24061 USA
关键词
ligament; fibroblast; electrospin; cell adhesion; cell proliferation; cell morphology;
D O I
10.1016/j.biomaterials.2006.07.005
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Engineered ligament tissues are promising materials for the repair of tears and ruptures, but require the development of biomaterial scaffolds that not only support physiologically relevant loads, but also possess architectures capable of orienting cell adhesion and extracellular matrix deposition. Based on evidence that micron-scale topographic features induce cell orientation through a contact guidance phenomenon, we postulate that oriented micron-scale fiber meshes-formed by the electrospinning process-can regulate cell morphology. To test this, fused fiber meshes of poly(D,L-lactic-co-glycolic acid) (PLGA) were electrospun onto rigid supports under conditions that produced mean fiber diameters of 0.14-3.6 mu m, and angular standard deviations of 31-60 degrees. Analysis of the morphology of adherent NIH 3T3 fibroblasts indicated that projected cell area and aspect ratio increased systematically with both increasing fiber diameter and degree of fiber orientation. Importantly, cell morphology on 3.6 mu m fibers was similar to that on spincoated PLGA films, Finally, cell densities on electrospun meshes were not significantly different from spincoated PLGA, indicating that cell proliferation is not sensitive to fiber diameter or orientation. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5681 / 5688
页数:8
相关论文
共 47 条
[1]   Silk matrix for tissue engineered anterior cruciate ligaments [J].
Altman, GH ;
Horan, RL ;
Lu, HH ;
Moreau, J ;
Martin, I ;
Richmond, JC ;
Kaplan, DL .
BIOMATERIALS, 2002, 23 (20) :4131-4141
[2]   Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates [J].
Badami, AS ;
Kreke, MR ;
Thompson, MS ;
Riffle, JS ;
Goldstein, AS .
BIOMATERIALS, 2006, 27 (04) :596-606
[3]   Utilizing acid pretreatment and electrospinning to improve biocompatibility of poly(glycolic acid) for tissue engineering [J].
Boland, ED ;
Telemeco, TA ;
Simpson, DG ;
Wnek, GE ;
Bowlin, GL .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2004, 71B (01) :144-152
[4]   Micropatterned surfaces for control of cell shape, position, and function [J].
Chen, CS ;
Mrksich, M ;
Huang, S ;
Whitesides, GM ;
Ingber, DE .
BIOTECHNOLOGY PROGRESS, 1998, 14 (03) :356-363
[5]   Fiber-based tissue-engineered scaffold for ligament replacement: design considerations and in vitro evaluation [J].
Cooper, JA ;
Lu, HH ;
Ko, FK ;
Freeman, JW ;
Laurencin, CT .
BIOMATERIALS, 2005, 26 (13) :1523-1532
[6]   Topographical control of cells [J].
Curtis, A ;
Wilkinson, C .
BIOMATERIALS, 1997, 18 (24) :1573-1583
[7]  
den Braber ET, 1998, J BIOMED MATER RES, V40, P291, DOI 10.1002/(SICI)1097-4636(199805)40:2<291::AID-JBM14>3.3.CO
[8]  
2-8
[9]   Guidance of engineered tissue collagen orientation by large-scale scaffold microstructures [J].
Engelmayr, George C., Jr. ;
Papworth, Glenn D. ;
Watkins, Simon C. ;
Mayer, John E., Jr. ;
Sacks, Michael S. .
JOURNAL OF BIOMECHANICS, 2006, 39 (10) :1819-1831
[10]  
Fisher N.I, 1995, STAT ANAL CIRCULAR D