Molecular cloning and characterization of hetR genes from filamentous cyanobacteria

被引：7

作者：

Schiefer, W ^{[1
]}

Schütz, K ^{[1
]}

Hachtel, W ^{[1
]}

Happe, T ^{[1
]}

机构：

[1] Univ Bonn, Inst Bot, D-53115 Bonn, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2002年 / 1577卷 / 01期

关键词：

cyanobacteria; heterocyst development; hetR; nitrogen fixation;

D O I：

10.1016/S0167-4781(02)00399-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HetR, a serine type protease, plays an important role in heterocyst differentiation in filamentous cyanobacteria. We isolated and sequenced the hetR genes from different heterocystous and filamentous nonheterocystous cyanobacteria. The hetR gene in the heterocyst forming Anabaena variabilis ATCC 29413 FD was interrupted by interposon mutagenesis (mutant strain WSIII8). This mutant does not form heterocysts and shows no diazotrophic growth under aerobic conditions. However, under anaerobic N-2-fixing conditions, the WSIII8 cells are able to grow, and high nitrogenase (Nif2) activity is detectable. Nif2 expression was demonstrated in each vegetative cell of the filament by immunolocalization 4 It after nitrogen step-down. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：139 / 143

页数：5

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