Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli

被引：55

作者：

Hebert, EJ

Grimsley, GR

Hartley, RW

Horn, G

Schell, D

Garcia, S

Both, V

Sevcik, J

Pace, CN

机构：

[1] TEXAS A&M UNIV, DEPT MED BIOCHEM & GENET, COLLEGE STN, TX 77843 USA

[2] TEXAS A&M UNIV, CTR MACROMOL DESIGN, COLLEGE STN, TX 77843 USA

[3] NIDDKD, CELLULAR & DEV BIOL LAB, NIH, BETHESDA, MD 20892 USA

[4] SLOVAK ACAD SCI, INST MOL BIOL, BRATISLAVA 84251, SLOVAKIA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1997年 / 11卷 / 02期

关键词：

D O I：

10.1006/prep.1997.0776

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes for three small ribonucleases from different strains of Streptomyces aureofaciens have been cloned and expressed in Escherichia coli. The purification of these ribonucleases from the periplasmic space is described, The yields range from 10 to 50 mg of protein per liter of culture medium. The molar absorption coefficients, isoelectric pH values, and pH of optimum activity are reported. (C) 1997 Academic Press.

引用

页码：162 / 168

页数：7

共 35 条

[1] The determinants of pK(a)s in proteins [J].