Cationic microparticles: A potent delivery system for DNA vaccines

被引：402

作者：

Singh, M ^{[1
]}

Briones, M ^{[1
]}

Ott, G ^{[1
]}

O'Hagan, D ^{[1
]}

机构：

[1] Chiron Corp, Emeryville, CA 94608 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 02期

关键词：

DNA delivery; poly(lactide-co-glycolide) microparticles;

D O I：

10.1073/pnas.97.2.811

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An approach involving the preparation of biodegradable microparticles with a cationic surface was developed to improve the delivery of adsorbed DNA into antigen-presenting cells after i.m. injection. The microparticles released intact and functional DNA over 2 weeks in vitro. In addition, the microparticles induced higher levels of marker gene expression in vivo. After i.m. immunization, the microparticles induced significantly enhanced serum antibody responses in comparison to naked DNA. Moreover, the level of antibodies induced by the microparticles was significantly enhanced by the addition of a vaccine adjuvant, aluminum phosphate. In addition, in contrast to naked DNA, the cationic microparticles induced potent cytotoxic T lymphocyte responses at a low dose.

引用

页码：811 / 816

页数：6

共 24 条

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