Aspergillus PCR -: Platforms, strengths and weaknesses

被引：23

作者：

White, P. Lewis

Barnes, Rosemary A.

机构：

[1] Univ Wales Hosp, Dept Med Microbiol, Cardiff CF14 4XN, Wales

[2] Univ Wales Hosp, NPHS Cardiff, Cardiff CF14 4XN, Wales

来源：

MEDICAL MYCOLOGY | 2006年 / 44卷

关键词：

sample type; DNA extraction; PCR amplification; result interpretation;

D O I：

10.1080/13693780600898003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

PCR is a useful tool to aid in the diagnosis of invasive aspergillosis. However, it is essential that an optimal method be agreed to allow inclusion in future consensus diagnosis criteria. It should be used in conjunction with other methods (e.g., galatomarman (GM) ELISA and high resolution computed tomography (HRCT)) to enhance the opportunity for detection of this devastating infection. This manuscript will try to highlight the benefits but mainly the limitations occurring throughout the process of molecular testing. It will focus on real-time methods although many of the points will be relevant to block-based amplification.

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收藏

页码：S191 / S198

页数：8

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