Cytochrome c folding pathway:: Kinetic native-state hydrogen exchange

被引：112

作者：

Hoang, L ^{[1
]}

Bédard, S ^{[1
]}

Krishna, MMG ^{[1
]}

Lin, Y ^{[1
]}

Englander, SW ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Johnson Res Fdn, Philadelphia, PA 19104 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷 / 19期

关键词：

protein folding; unfolding rollover; alkaline transition;

D O I：

10.1073/pnas.152439199

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.

引用

页码：12173 / 12178

页数：6

共 43 条

[1] MECHANISM OF REDUCTION OF SINGLE-SITE REDOX PROTEINS BY ASCORBIC-ACID [J].

ALAYASH, AI ;

WILSON, MT .

BIOCHEMICAL JOURNAL, 1979, 177 (02) :641-648

[2] Microsecond to minute dynamics revealed by EX1-type hydrogen exchange at nearly every backbone hydrogen bond in a native protein [J].

Arrington, CB ;

Robertson, AD .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 296 (05) :1307-1317

[3] PRIMARY STRUCTURE EFFECTS ON PEPTIDE GROUP HYDROGEN-EXCHANGE [J].

BAI, YW ;

MILNE, JS ;

MAYNE, L ;

ENGLANDER, SW .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 17 (01) :75-86

[4] Kinetic evidence for an on-pathway intermediate in the folding of cytochrome c [J].

Bai, YW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (02) :477-480

[5]

Bai YW, 1996, PROTEINS, V24, P145, DOI 10.1002/(SICI)1097-0134(199602)24:2<145::AID-PROT1>3.0.CO

[6]

2-I

[7] PROTEIN STABILITY PARAMETERS MEASURED BY HYDROGEN-EXCHANGE [J].

BAI, YW ;

MILNE, JS ;

MAYNE, L ;

ENGLANDER, SW .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 1994, 20 (01) :4-14

[8] PROTEIN-FOLDING INTERMEDIATES - NATIVE-STATE HYDROGEN-EXCHANGE [J].

BAI, YW ;

SOSNICK, TR ;

MAYNE, L ;

ENGLANDER, SW .

SCIENCE, 1995, 269 (5221) :192-197

[9] Equilibrium amide hydrogen exchange and protein folding kinetics [J].

Bai, YW .

JOURNAL OF BIOMOLECULAR NMR, 1999, 15 (01) :65-70

[10]

BERGER ARIEH, 1957, ARCH BIOCHEM AND BIOPHYS, V69, P106, DOI 10.1016/0003-9861(57)90478-2

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