Effect of a C/EBP gene replacement on mitochondrial biogenesis in fat cells

被引：74

作者：

Chin, CH ^{[1
]}

Lin, WD ^{[1
]}

Huang, SY ^{[1
]}

Lee, YH ^{[1
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Lab Mol Pathol, Taipei 115, Taiwan

来源：

GENES & DEVELOPMENT | 2004年 / 18卷 / 16期

关键词：

C/EBP; gene replacement; G alpha(s); WAT adipocyte; mitochondrial biogenesis;

D O I：

10.1101/gad.1213104

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

CCAAT/enhancer-binding proteins, C/EBPalpha and C/EBPbeta, are required for fat cell differentiation and maturation. Previous studies showed that replacement of C/EBPalpha with C/EBPbeta, generating the beta/beta alleles in the mouse genome, prevents lipid accumulation in white adipose tissue (WAT). in this study, beta/beta mice lived longer and had higher energy expenditure than their control littermates due to increased WAT energy oxidation. The WAT of beta/beta mice was enriched with metabolically active, thermogenic mitochondria known for energy burning. The beta/beta allele exerted its effect through the elevated expression of the G protein alpha stimulatory subunit (Galpha(s)) in WAT. Galpha(s), when overexpressed in fat-laden 3T3-L1 cells, stimulated mitochondrial biogenesis similar to that seen in the WAT of beta/beta mice, and effectively diminished the stored lipid pool.

引用

页码：1970 / 1975

页数：6

共 40 条

[1] β3-adrenoceptor agonists:: potential, pitfalls and progress [J].

Arch, JRS .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2002, 440 (2-3) :99-107

[2] Uncoupling protein-3: A new member of the mitochondrial carrier family with tissue-specific expression [J].

Boss, O ;

Samec, S ;

PaoloniGiacobino, A ;

Rossier, C ;

Dulloo, A ;

Seydoux, J ;

Muzzin, P ;

Giacobino, JP .

FEBS LETTERS, 1997, 408 (01) :39-42

[3] C/EBPβ, when expressed from the C/ebpα gene locus, can functionally replace C/EBPα in liver but not in adipose tissue [J].

Chen, SS ;

Chen, JF ;

Johnson, PF ;

Muppala, V ;

Lee, YH .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (19) :7292-7299

[4] The β-adrenergic receptors and the control of adipose tissue metabolism and thermogenesis [J].

Collins, S ;

Surwit, RS .

RECENT PROGRESS IN HORMONE RESEARCH, VOL 56, 2001, 56 :309-328

[5]

COOPERSTEIN SJ, 1951, J BIOL CHEM, V189, P665

[6] Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries [J].

Diatchenko, L ;

Lau, YFC ;

Campbell, AP ;

Chenchik, A ;

Moqadam, F ;

Huang, B ;

Lukyanov, S ;

Lukyanov, K ;

Gurskaya, N ;

Sverdlov, ED ;

Siebert, PD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6025-6030

[7] CCAAT/enhancer-binding protein α is required for transcription of the β3-adrenergic receptor gene during adipogenesis [J].

Dixon, TM ;

Daniel, KW ;

Farmer, SR ;

Collins, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (01) :722-728

[8] Interaction with Gβγ is required for membrane targeting and palmitoylation of Gαs, and Gαq [J].

Evanko, DS ;

Thiyagarajan, MM ;

Wedegaertner, PB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (02) :1327-1336

[9] Uncoupling protein-2: A novel gene linked to obesity and hyperinsulinemia [J].

Fleury, C ;

Neverova, M ;

Collins, S ;

Raimbault, S ;

Champigny, O ;

LeviMeyrueis, C ;

Bouillaud, F ;

Seldin, MF ;

Surwit, RS ;

Ricquier, D ;

Warden, CH .

NATURE GENETICS, 1997, 15 (03) :269-272

[10] THE ADIPOCYTE - STORAGE DEPOT OR NODE ON THE ENERGY INFORMATION SUPERHIGHWAY [J].

FLIER, JS .

CELL, 1995, 80 (01) :15-18

← 1 2 3 4 →