Mitochondrial DNA content in paired normal and cancerous breast tissue samples from patients with breast cancer

被引：74

作者：

Fan, Alex Xiu-Cheng ^{[1
]}

Radpour, Ramin ^{[1
]}

Haghighi, Mahdi Montazer ^{[2
]}

Kohler, Corina ^{[1
]}

Xia, Peng ^{[1
]}

Hahn, Sinuhe ^{[1
]}

Holzgreve, Wolfgang ^{[1
,3
]}

Zhong, Xiao Yan ^{[1
]}

机构：

[1] Univ Basel, Womens Hosp, Dept Biomed, Lab Prenatal Med & Gynecol Oncol, CH-4031 Basel, Switzerland

[2] Azad Univ, E Tehran Branch, Tehran, Iran

[3] Univ Freiburg, Univ Med Ctr, D-79106 Freiburg, Germany

来源：

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | 2009年 / 135卷 / 08期

基金：

瑞士国家科学基金会;

关键词：

Quantitative alteration; Mitochondrial DNA; Breast cancer; Breast tissue; PROSTATE-CANCER; COPY NUMBER; MUTATIONS; RECEPTORS; DELETION; CELLS; HEAD;

D O I：

10.1007/s00432-008-0533-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We develop a multiplex quantitative real-time PCR for synchronized analysis of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) to investigate relative mtDNA abundance in paired normal and cancerous breast tissues. The amounts of nDNA and mtDNA in 102 tissue samples were quantified for both glyceraldehype-3-phosphodehydrogenase (GAPDH) gene and mtDNA encoded ATPase (MTATP) 8 gene. The average threshold cycle (Ct) number values of the nDNA and mtDNA were used to calculate relative mtDNA content in breast tissues. The median delta Ct (Delta Ct) and the median mtDNA content for normal and cancerous breast tissues were 6.73 and 2.54, as well as 106.50 and 5.80 (P = 0.000, respectively). The mtDNA content was decreased in 82% of cancerous breast tissues compared with the normal ones. The changes were associated with hormone receptor status. Our finding suggests that decreased mtDNA content in breast cancer may have diagnostic and prognostic value for the disease.

引用

页码：983 / 989

页数：7

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