The yeast SR protein kinase Sky1p modulates salt tolerance, membrane potential and the Trk1,2 potassium transporter

被引:41
作者
Forment, J [1 ]
Mulet, JM [1 ]
Vicente, O [1 ]
Serrano, R [1 ]
机构
[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Dept Biotechnol, Valencia 46022, Spain
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2002年 / 1565卷 / 01期
关键词
Saccharomyces cerevisiae; ion homeostasis; protein kinase; salt tolerance; potassium transport; membrane potential;
D O I
10.1016/S0005-2736(02)00503-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein kinases dedicated to the phosphorylation of SR proteins have been implicated in the processing and nuclear export of mRNAs. Here we demonstrate in Saccharomyces cerevisiae their participation in cation homeostasis. A null mutant of the single yeast SR protein kinase Sky1p is viable but exhibits increased tolerance to diverse toxic cations such as Na+, Li+, spermine, tetramethylammonium, hygromycin B and Mn2+. This pleiotropic phenotype correlates with reduced accumulation of cations, suggesting a decrease in membrane electrical potential. Genetic analysis and Rb+ uptake measurements indicate that Sky1p modulates Trk 1,2, the high-affinity K+ uptake system of yeast and a major determinant of membrane potential. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:36 / 40
页数:5
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