Gene expression in epithelial ovarian carcinoma

被引：85

作者：

Matei, D

Graeber, TG

Baldwin, RL

Karlan, BY

Rao, JY

Chang, DD

机构：

[1] Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Sch Med, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Dept Energy, Lab Struct Biol & Mol Med, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Sch Med, Dept Obstet & Gynecol, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

[6] Cedars Sinai Med Ctr, Div Gynecol Oncol, Los Angeles, CA 90048 USA

来源：

ONCOGENE | 2002年 / 21卷 / 41期

关键词：

ovarian epithelial cells; oligonucleotide arrays; RT-PCR; periostin;

D O I：

10.1038/sj.onc.1205785

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analysed the mRNA levels corresponding to 12 600 transcripts in primary cultures of ovarian epithelial cells derived from nine normal ovaries and 21 epithelial ovarian carcinoma. The class distinction and hierarchical clustering of expression data revealed a clear distinction in gene expression between normal and carcinoma-derived ovarian epithelial cells. Comparison of expression levels revealed 111 genes with mean expression values of >2.5-fold higher in carcinoma cells. Similarly, 62 genes were expressed at >2.5-fold higher levels in normal ovarian epithelial cells. For a few selected genes, we demonstrate that the pattern of differential expression observed in cultured epithelial cells is present in the normal ovaries and epithelial ovarian carcinoma. Use of cultured epithelial cells represents a novel strategy to study gene expression in a cell-type specific manner.

引用

页码：6289 / 6298

页数：10

共 46 条

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