Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry

被引：12

作者：

Fu, Guo ^{[1
]}

Gascoigne, Nicholas R. J. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Immunol & Microbial Sci, IMM1, La Jolla, CA 92037 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 350卷 / 1-2期

关键词：

Calcium flux; CFSE; Cy5; Lymphocyte activation; LYMPHOCYTE-PROLIFERATION;

D O I：

10.1016/j.jim.2009.07.009

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Calcium flux measurement is a crucial assay in lymphocyte activation. However, with the currently well established flow cytometric methods, it is a tedious procedure that is difficult to control to avoid variation between samples. This leads to unwanted sources of error that can make it problematic to interpret the results. Here we present an improved method that allows different cell populations to be tested in the same sample. Samples are pre-labeled with CFSE or Cy5 then mixed and stimulated to induce calcium flux. This facilitates more rapid and accurate measurement of calcium flux and also dramatically reduces the cost and effort required for this type of assay. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：194 / 199

页数：6

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