Rapid and quantitative detection of Brucella by up-converting phosphor technology-based lateral-flow assay

被引：82

作者：

Qu, Qing ^{[2
,3
]}

Zhu, Ziwen ^{[1
]}

Wang, Yufei ^{[2
]}

Zhong, Zhijun ^{[2
]}

Zhao, Jin ^{[2
]}

Qiao, Feng ^{[2
]}

Du, Xinying ^{[2
]}

Wang, Zhoujia ^{[2
]}

Yang, Ruifu ^{[1
]}

Huang, Liuyu ^{[2
]}

Yu, Yaqin ^{[3
]}

Zhou, Lei ^{[1
]}

Chen, Zeliang ^{[2
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Lab Analyt Microbiol, Beijing 100071, Peoples R China

[2] Beijing Inst Dis Control & Prevent, Dept Infect Dis Control, Beijing 100071, Peoples R China

[3] Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 79卷 / 01期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Up-converting phosphor technology; Immunoassay; Quantitative detection; Brucella; AMPLIFICATION; IMMUNOASSAY; PATHOGENS;

D O I：

10.1016/j.mimet.2009.07.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and quantitative up-converting phosphor technology-based later-flow assay (UPT-LF assay) was developed for on-site detection of Brucella. Different Brucella species both in pure cultures and in spiked samples could be quantitatively detected. The detection limit for pure culture was 5 x 10(6) CFU/ml and the sensitivity for different spiked samples ranged from 2.0 x 10(3) to 3.9 x 10(5) CFU/mg. The UPT-LF assay showed high specificity. reproducibility and stability, providing great potential for Brucella on-site detection. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：121 / 123

页数：3

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