Wolbachia Mediate Variation of Host Immunocompetence

被引:63
作者
Braquart-Varnier, Christine [1 ]
Lachat, Marion [1 ]
Herbiniere, Juline [1 ]
Johnson, Monique [1 ]
Caubet, Yves [1 ]
Bouchon, Didier [1 ]
Sicard, Mathieu [1 ]
机构
[1] Univ Poitiers, Ecol Lab, CNRS, UMR 6556, Poitiers, France
来源
PLOS ONE | 2008年 / 3卷 / 09期
关键词
D O I
10.1371/journal.pone.0003286
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: After decades during which endosymbionts were considered as silent in their hosts, in particular concerning the immune system, recent studies have revealed the contrary. In the present paper, we addressed the effect of Wolbachia, the most prevalent endosymbiont in arthropods, on host immunocompetence. To this end, we chose the A. vulgare-Wolbachia symbiosis as a model system because it leads to compare consequences of two Wolbachia strains (wVulC and wVulM) on hosts from the same population. Moreover, A. vulgare is the only host-species in which Wolbachia have been directly observed within haemocytes which are responsible for both humoral and cellular immune responses. Methodology/Principal Findings: We sampled gravid females from the same population that were either asymbiotic, infected with wVulC, or infected with wVulM. The offspring from these females were tested and it was revealed that individuals harbouring wVulC exhibited: (i) lower haemocyte densities, (ii) more intense septicaemia in their haemolymph and (iii) a reduced lifespan as compared to individuals habouring wVulM or asymbiotic ones. Therefore, individuals in this population of A. vulgare appeared to suffer more from wVulC than from wVulM. Symbiotic titer and location in the haemocytes did not differ for the two Wolbachia strains showing that these two parameters were not responsible for differences observed in their extended phenotypes in A. vulgare. Conclusion/Significance: The two Wolbachia strains infecting A. vulgare in the same population induced variation in immunocompetence and survival of their hosts. Such variation should highly influence the dynamics of this host-symbiont system. We propose in accordance with previous population genetic works, that wVulM is a local strain that has attenuated its virulence through a long term adaptation process towards local A. vulgare genotypes whereas wVulC, which is a widespread and invasive strain, is not locally adapted.
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相关论文
共 38 条
[1]   Wolbachia surface protein (WSP) inhibits apoptosis in human neutrophils [J].
Bazzocchi, C. ;
Comazzi, S. ;
Santoni, R. ;
Bandi, C. ;
Genchi, C. ;
Mortarino, M. .
PARASITE IMMUNOLOGY, 2007, 29 (02) :73-79
[2]   Evidence for widespread Wolbachia infection in isopod crustaceans:: molecular identification and host feminization [J].
Bouchon, D ;
Rigaud, T ;
Juchault, P .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1998, 265 (1401) :1081-1090
[3]   The Endosymbiont Wolbachia pipientis Induces the Expression of Host Antioxidant Proteins in an Aedes albopictus Cell Line [J].
Brennan, Lesley J. ;
Keddie, B. Andrew ;
Braig, Henk R. ;
Harris, Harriet L. .
PLOS ONE, 2008, 3 (05)
[4]   Evidence for a new feminizing Wolbachia strain in the isopod Armadillidium vulgare:: evolutionary implications [J].
Cordaux, R ;
Michel-Salzat, A ;
Frelon-Raimond, M ;
Rigaud, T ;
Bouchon, D .
HEREDITY, 2004, 93 (01) :78-84
[5]   Antibiotic curing of parthenogenesis in Eretmocerus mundus (Australian parthenogenic form) [J].
De Barro, PJ ;
Hart, PJ .
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, 2001, 99 (02) :225-230
[6]   A Wolbachia-associated fitness benefit depends on genetic background in Drosophila simulans [J].
Dean, Matthew D. .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2006, 273 (1592) :1415-1420
[7]   Removing symbiotic Wolbachia bacteria specifically inhibits oogenesis in a parasitic wasp [J].
Dedeine, F ;
Vavre, F ;
Fleury, F ;
Loppin, B ;
Hochberg, ME ;
Boulétreau, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (11) :6247-6252
[8]  
Dobson SL, 2002, GENETICS, V160, P1087
[9]  
Dong P, 2007, J ECON ENTOMOL, V100, P1476, DOI 10.1603/0022-0493(2007)100[1476:IOWIOT]2.0.CO
[10]  
2