An in vivo analysis of cytokine production during Leishmania donovani infection in scid Mice

被引:46
作者
Engwerda, CR
Smelt, SC
Kaye, PM
机构
[1] Immunology and Cell Biology Unit, Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, Keppel Street
基金
英国惠康基金;
关键词
Leishmania donovani; cytokine mRNA; scid mice; liver; granulomas; TNF alpha;
D O I
10.1006/expr.1996.0105
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Leishmania donovani, the causative agent of visceral leishmaniasis, fails to induce NK cell activation in scid mice. In order to further our understanding of the host response to L. donovani, we analysed cytokine mRNA accumulation and TNF alpha protein synthesis in the liver of scid and BALB/c mice infected with this parasite. scid mice infected with L. donovani exhibited very little proinflammatory response, as measured by cytokine mRNA accumulation and TNF alpha protein expression, supporting the notion of a relatively ''quiet'' interaction between L. donovani and macrophages in these animals. In contrast, immunocompetent BALB/c mice were found to generate an early IFN gamma response, coincident with a rise in IL-2 mRNA levels and elaboration of a tissue response involving TNF alpha production by infected Kupffer cells. These results extend our understanding of the response of BALB/c and scid mice to L. donovani infection and highlight the value of cytokine analysis at both the tissue and cellular levels. (C) 1996 Academic Press, Inc.
引用
收藏
页码:195 / 202
页数:8
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