Death receptor 3 (DR3) gene duplication in a chromosome region 1p36.3: gene duplication is more prevalent in rheumatoid arthritis

被引：30

作者：

Osawa, K

Takami, N

Shiozawa, K

Hashiramoto, A

Shiozawa, S

机构：

[1] Kobe Univ, FHS Sch Med, Dept Rheumatol, Suma Ku, Kobe, Hyogo 6540142, Japan

[2] Kobe Univ, Grad Sch Med, Div Bioregulat, Kobe, Hyogo, Japan

[3] Konan Kakogawa Hosp, Dept Rheumatol, Kakogawa, Japan

[4] Kobe Univ Hosp, Div Rheumat Dis, Kobe, Hyogo, Japan

[5] COE, Shizuoka, Japan

来源：

GENES AND IMMUNITY | 2004年 / 5卷 / 06期

关键词：

gene duplication; death receptor 3 (DR3); fluorescence in situ hybridization (FISH); rheumatoid arthritis (RA);

D O I：

10.1038/sj.gene.6364097

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

The death receptor 3 (DR3) gene is a member of the apoptosis-inducing Fas gene family. In the current study, fluorescence in situ hybridization (FISH) and Fiber-FISH revealed the existence of a second DR3 gene similar to200 kb upstream of the original DR3 gene. The existence of the duplicated DR3 gene was confirmed by sequencing the corresponding human artificial chromosome clones as well as with quantitative PCR that measured the ratio of the DR3 gene mutation (Rm), intrinsic to rheumatoid arthritis ( RA) patients, by simultaneous amplification of the normal and mutated DR3 sequences. The DR3 gene duplication measured by FISH was found to be more frequent in patients with RA as compared to healthy individuals. We therefore surmise that the human DR3 gene can be duplicated and that this gene duplication is more prevalent in patients with RA.

引用

页码：439 / 443

页数：5

共 19 条

[1]

THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS [J].