Generation and use of alternative multimers of peptide/MHC complexes

被引：23

作者：

Hugues, S ^{[1
]}

Malherbe, L ^{[1
]}

Filippi, C ^{[1
]}

Glaichenhaus, N ^{[1
]}

机构：

[1] CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2002年 / 268卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

Major Histocompatibility Complex (MHC); multimers; tetramers; oligomers; T lymphocytes;

D O I：

10.1016/S0022-1759(02)00202-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For many years, the detection of antigen-specific T cells has relied on indirect in vitro assays such as cytokine secretion, proliferation or chromium release assays. Things have dramatically changed during the past few years, thanks to the imagination of several investigators who have developed very elegant strategies to produce multivalent peptide/MHC complexes. One of these strategies has been to produce peptide-loaded monomeric biotinylated MHC molecules, which could be obtained as tetramers upon incubation with tetravalent streptavidin. Although this latter approach has been by far the most popular, this review focuses on other strategies which have also been successful. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：83 / 92

页数：10

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