Centromeric behaviour in wheat with high and low homoeologous chromosomal pairing

被引:43
作者
AragonAlcaide, L [1 ]
Reader, S [1 ]
Miller, T [1 ]
Moore, G [1 ]
机构
[1] JOHN INNES CTR,NORWICH NR4 7UH,NORFOLK,ENGLAND
关键词
D O I
10.1007/s004120050254
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Control of homoeologous chromosomal pairing in hexaploid wheat stems from a balance between a number of suppressor and promoter genes. This study used centromeric behaviour as a tool to investigate the mechanism. Fluorescent in situ hybridization employing centromeric and telomeric sequences as probes was applied to pollen mother cells of wheat and wheat/alien hybrids having different pairing gene combinations. It showed: association of centromeres during pre-meiotic interphase; decondensation of centromeric structure; sister chromatid disjunction of univalent chromosomes in homoeologous pairing situations at anaphase I; and centromeric stretching between univalent sister chromatids in wheat/rye hybrids deficient for pairing genes. The implications of these results are discussed.
引用
收藏
页码:327 / 333
页数:7
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