Characterization of the chondroitin sulfates in wild type Caenorhabditis elegans

被引：7

作者：

Beeber, C

Kieras, FJ

机构：

[1] New York State Inst Basic Res Dev Disabil, Dept Human Genet, Staten Isl, NY 10314 USA

[2] CUNY, Kingsborough Community Coll, Dept Biol Sci, Brooklyn, NY 11235 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 05期

关键词：

glycosaminoglycan (GAG); proteoglycan (PG); Caenorhabditis elegans; thin layer chromatography (TLC);

D O I：

10.1016/S0006-291X(02)00399-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this study was to isolate and characterize the GAGs from the wild type nematode Caenorhabditis elegans in preparation for the characterization of the transgenic form constructed by Link [Proc. Natl. Acad. Sci. USA 92 (1995) 9368] which expresses various forms of P-peptide (or A4 peptide). This peptide forms deposits very similar to the ones found in the neuritic plaques and neurofibrillary tangles in Alzheimer disease (AD). Characterization has been accomplished by degradation with specific enzymes and analysis of the products by TLC and HPLC. The results were compared with earlier works and shown to differ in disaccharide content. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：1374 / 1376

页数：3

共 12 条

[1]

DAMLE SP, 1979, J BIOL CHEM, V254, P1614

[2] SELECTIVE URINARY SCREENING FOR MUCOPOLYSACCHARIDOSES [J].