Host-Derived Loss of Dentin Matrix Stiffness Associated With Solubilization of Collagen

被引:146
作者
Carrilho, Marcela R. [1 ,2 ]
Tay, Franklin R. [1 ,3 ]
Donnelly, Adam M. [1 ]
Agee, Kelli A. [1 ]
Tjaederhane, Leo [4 ,5 ]
Mazzoni, Annalisa [6 ]
Breschi, Lorenzo [7 ,8 ]
Foulger, Stephen [9 ]
Pashley, David H. [1 ]
机构
[1] Med Coll Georgia, Sch Dent, Dept Oral Biol, Augusta, GA 30912 USA
[2] Univ Estadual Campinas, Piracicaba Sch Dent, Dept Restorat Dent, Piracicaba, SP, Brazil
[3] Med Coll Georgia, Sch Dent, Dept Endodont, Augusta, GA 30912 USA
[4] Univ Oulu, Inst Dent, Oulu, Finland
[5] Oulu Univ Hosp, Oulu, Finland
[6] Univ Bologna, Dept SAU & FAL, Bologna, Italy
[7] Univ Trieste, Dept Biomed, Div Dent Sci & Biomat, Trieste, Italy
[8] IOR, IGM CNR, Unit Bologna, Bologna, Italy
[9] Clemson Univ, Dept Mat Sci Engn, Clemson, SC USA
基金
巴西圣保罗研究基金会;
关键词
dentin collagen; electron microscopy; matrix metalloproteinase; mechanical properties; MACROMOLECULAR ORGANIZATION; DENATURATION TEMPERATURES; ELECTRON-MICROSCOPY; TENSILE-STRENGTH; CROSS-LINKING; HYBRID LAYER; IN-VIVO; DEGRADATION; RESIN; METALLOPROTEINASES;
D O I
10.1002/jbm.b.31295
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Matrix metalloproteinases (MMPs) bound to dentin matrices are activated during adhesive bonding procedures and are thought to contribute to the progressive degradation of resin-dentin bonds over time. The purpose of this study was to evaluate the changes in mechanical, biochemical, and structural properties of demineralized dentin treated with or without chlorhexidine (CHX), a known MMP-inhibitor. After demineralizing dentin beams in EDTA or phosphoric acid (PA), the baseline modulus of elasticity (E) of each beam was measured by three-point flexure. Specimens were pretreated with water (control) or with 2% CHX (experimental) and then incubated in artificial saliva (AS) at 37 degrees C for 4 weeks. The E of each specimen was remeasured weekly and, the media was analyzed for solubilized dentin collagen at first and fourth week of incubation. Some specimens were processed for electron microscopy (TEM) immediately after demineralization and after 4 weeks of incubation. In EDTA and PA-demineralized specimens, the E of the control specimens fell (p < 0.05) after incubation in AS, whereas there were no changes in E of the CHX-pretreated specimens over time. More collagen was solubilized from PA-demineralized controls (p < 0.05) than from EDTA-demineralized matrices after I or 4 weeks. Less collagen (p < 0.05) was solubilized from CHX-pretreated specimens demineralized in EDTA compared with PA. TEM examination of control beams revealed that prolonged demineralization of dentin in 10% PA (12 h) did not denature the collagen fibrils. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 373-380, 2009
引用
收藏
页码:373 / 380
页数:8
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