Electrochemical study of S-nitrosoglutathione and nitric oxide by carbon fibre NO sensor and cyclic voltammetry -: possible way of monitoring of nitric oxide

被引:39
作者
Vitecek, Jan
Petrlova, Jitka
Petrek, Jiri
Adam, Vojtech
Potesil, David
Havel, Ladislav
Mikelova, Radka
Trnkova, Libuse
Kizek, Rene
机构
[1] Mendel Univ Agr & Forestry, Dept Chem & Biochem, Brno 61300, Czech Republic
[2] Mendel Univ Agr & Forestry, Dept Plant Biol, Brno 61300, Czech Republic
[3] Masaryk Univ, Fac Sci, Dept Analyt Chem, CS-61137 Brno, Czech Republic
[4] Masaryk Univ, Fac Sci, Dept Theoret & Phys Chem, CS-61137 Brno, Czech Republic
关键词
glutathione; GSH; GSSG; nitrosoglutathione; S-nitroso-N-acety-D; L-penicillamine; thiols; nitric oxide; cyclic voltammetry; carbon fibre electrode; copper; iron;
D O I
10.1016/j.electacta.2006.03.079
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Nitrosation of sulfhydryl group of glutathione, which is highly reactive and is often found conjugated to other molecules via its sulfhydryl moiety, is one of many biological effects of the nitric oxide (NO). This process may serve as a signal event and/or as a deposition of NO to S-nitrosoglutathione (GSNO). Moreover, GSNO may release NO under specific conditions. That is why NO, which has a little lifetime itself, could be distribute for longer distances within the organism. Here, we studied and compared the basic electrochemical characteristics of biological active thiol compounds (GSH, oxidized glutathione and GSNO). In addition, observation of the decomposition process of GSNO using different electrochemical techniques followed. Primarily we studied the influence of scan rate and reducing agent (Tris(2-carboxyethyl)phosphine). The CV calibration equations were linear, R.S.D. about 5%. The detection limits of GSH, GSSG and GSNO expressed as 3 S/N were 9 nM, 4 nM and 20 nM, respectively. In addition, the use of NO selective carbon fibre electrode and cyclic voltammetry for the study of GSNO decomposition catalysed by copper(II) and iron(II) followed. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5087 / 5094
页数:8
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