Oligonucleotide-conjugated beads for transdominant genetic experiments

被引：12

作者：

Feldhaus, MJ ^{[1
]}

Lualhati, M ^{[1
]}

Cardon, K ^{[1
]}

Roth, B ^{[1
]}

Kamb, A ^{[1
]}

机构：

[1] Arcaris Inc, Salt Lake City, UT 84108 USA

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 02期

关键词：

D O I：

10.1093/nar/28.2.534

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division, In conjunction with these libraries, oligonucleotide-conjugated beads and flow cytometry were used to test a strategy that potentially expands the range of such genetic studies, The experimental approach involved creation of tagged expression libraries, introduction of these libraries into cells, growth of the cultured cells for several generations and recovery on oligonucleotide-conjugated beads of sequences that encode growth-modulatory proteins or peptides, Experiments in Saccharomyces cerevisiae demonstrating the feasibility of the strategy are presented.

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收藏

页码：534 / 543

页数：10

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