A novel Nanoformulation Development of Eugenol and their treatment in inflammation and periodontitis

Objective: To prepare a novel nanoemulsion- Carbopol (R) 934 gel for Eugenol, in order to prevent the periodontitis. Material and methods: Spontaneous emulsification method was used for the preparation of nanoemulsion in which it contain Eugenol (oil phase), Tween-80 (surfactant), and PEG (co-surfactant). To the development of best nanoemulsion, three-factor three-level central composite design was used in which %oil; %S-mix and %water were optimized as independent variables. An optimized-nanoemulsion were converted to nanoemulsion-Carbopol (R) 934 gel. Results: 5.5% oil, 35.5% S-mix and 59.0% water were optimized as independent and dependent variables. Finally dependent variables optimized as a particle size (nm), PDI and %transmittance were observed 79.92 +/- 6.33 nm, 0.229 +/- 0.019, and 98.88 +/- 1.31% respectively. The values of final results for dependent variables like particle size (nm), PDI and % transmittance were evaluated as 79.92 +/- 6.33 nm, 0.229 +/- 0.019, and 98.88 +/- 1.31%, respectively. TEM and SEM showed a spherical shape of developed nanoemulsion with refractive index (1.63 +/- 0.038), zeta potential (-19.16 +/- 0.11), pH (7.4 +/- 0.06), viscosity (34.28 +/- 6 cp), and drug content of 98.8 +/- 0.09%. After that a final optimized EUG-NE-Gel was assessed on the basis of their pH measurement, drug content, syringeability, and mucoadhesion on the goat buccal mucosa. Optimized EUG-NE-Gel (Tween-80 and Carbopol (R) 934 used) showed the results, to improve the periodontal drug delivery of EUG in future. Conclusion: EUG-NE-Gel showed a significant role in anti-inflammatory activity, analgesic, and anesthetic, antibacterial, and treatment of periodontal disease. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.