Pyrrolysine encoded by UAG in Archaea: Charging of a UAG-decoding specialized tRNA

被引:460
作者
Srinivasan, G [1 ]
James, CM [1 ]
Krzycki, JA [1 ]
机构
[1] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
关键词
D O I
10.1126/science.1069588
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.
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收藏
页码:1459 / 1462
页数:4
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