Pyrrolysine encoded by UAG in Archaea: Charging of a UAG-decoding specialized tRNA

被引：460

作者：

Srinivasan, G ^{[1
]}

James, CM ^{[1
]}

Krzycki, JA ^{[1
]}

机构：

[1] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA

来源：

SCIENCE | 2002年 / 296卷 / 5572期

关键词：

D O I：

10.1126/science.1069588

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pyrrolysine is a lysine derivative encoded by the UAG codon in methylamine methyltransferase genes of Methanosarcina barkeri. Near a methyltransferase gene cluster is the pylT gene, which encodes an unusual transfer RNA (tRNA) with a CUA anticodon. The adjacent pylS gene encodes a class II aminoacyl-tRNA synthetase that charges the pylT-derived tRNA with lysine but is not closely related to known lysyl-tRNA synthetases. Homologs of pylS and pylT are found in a Gram-positive bacterium. Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid.

引用

页码：1459 / 1462

页数：4

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