A complex prediction:: three-dimensional model of the yeast exosome

被引：74

作者：

Aloy, P

Ciccarelli, FD

Leutwein, C

Gavin, AC

Superti-Furga, G

Bork, P

Böttcher, B

Russell, RB

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] Cellzome AG, D-69117 Heidelberg, Germany

来源：

EMBO REPORTS | 2002年 / 3卷 / 07期

关键词：

D O I：

10.1093/embo-reports/kvf135

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a model of the yeast exosome based on the bacterial degradosome component polynucleotide phosphorylase (PNPase). Electron microscopy shows the exosome to resemble PNPase but with key differences likely related to the position of RNA binding domains, and to the location of domains unique to the exosome. We use various techniques to reduce the many possible models of exosome subunits based on PNPase to just one. The model suggests numerous experiments to probe exosome function, particularly with respect to subunits making direct atomic contacts and conserved, possibly functional residues within the predicted central pore of the complex.

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收藏

页码：628 / 635

页数：8

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