Novel complex sul1-type integron in Escherichia coli carrying blaCTX-M-9

被引:78
作者
Sabaté, M
Navarro, F
Miró, E
Campoy, S
Mirelis, B
Barbé, J
Prats, G
机构
[1] Univ Autonoma Barcelona, Hosp Santa Creu & St Pau, Dept Microbiol, Barcelona 08025, Spain
[2] Univ Autonoma Barcelona, Dept Genet & Microbiol, E-08193 Barcelona, Spain
[3] Ctr Recerca Sanitat Anim, Bellaterra 08193, Spain
关键词
D O I
10.1128/AAC.46.8.2656-2661.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
For the present report, a novel complex class 1 integron, In60, was characterized. Part of this integron includes the bla(CTX-M-9) gene and its downstream nucleotide sequence, which shares 81% and 78% nucleotide identity with those of kluA-1 beta-lactamase and orf3 of K. ascorbata, respectively. Furthermore, a new insertion sequence, IS3000, has been found in In60. PCR analysis indicates that integron In60 is present in 33 of 34 nonclonal enterobacterial isolates carrying the putative beta-lactamase CTX-M-9.
引用
收藏
页码:2656 / 2661
页数:6
相关论文
共 18 条
[1]   REVISED ANALYSIS OF AADA2 GENE OF PLASMID PSA [J].
BITO, A ;
SUSANI, M .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (05) :1172-1175
[2]   Plasmid-mediated florfenicol resistance encoded by the floR gene in Escherichia coli isolated from cattle [J].
Cloeckaert, A ;
Baucheron, S ;
Flaujac, G ;
Schwarz, S ;
Kehrenberg, C ;
Martel, JL ;
Chaslus-Dancla, E .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (10) :2858-2860
[3]   Identification of the Rhodobacter sphaeroides SOS box [J].
de Henestrosa, ARF ;
Rivera, E ;
Tapias, A ;
Barbé, J .
MOLECULAR MICROBIOLOGY, 1998, 28 (05) :991-1003
[4]   Characterization of a chromosomally encoded extended-spectrum class A β-lactamase from Kluyvera cryocrescens [J].
Decousser, JW ;
Poirel, L ;
Nordmann, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (12) :3595-3598
[5]   Antibiotic resistance in gram-negative bacteria: the role of gene cassettes and integrons [J].
Hall, RM ;
Collis, CM .
DRUG RESISTANCE UPDATES, 1998, 1 (02) :109-119
[6]   THE STRUCTURE OF A PARTIAL DUPLICATION IN THE INTEGRON OF PLASMID PDGO100 [J].
HALL, RM ;
STOKES, HW .
PLASMID, 1990, 23 (01) :76-79
[7]   Integrating DNA: Transposases and retroviral integrases [J].
Haren, L ;
Ton-Hoang, B ;
Chandler, M .
ANNUAL REVIEW OF MICROBIOLOGY, 1999, 53 :245-281
[8]   The transposable elements resident on the plasmids of Pseudomonas putida strain H, Tn5501 and Tn5502, are cryptic transposons of the Tn3 family [J].
Lauf, U ;
Müller, C ;
Herrmann, H .
MOLECULAR AND GENERAL GENETICS, 1998, 259 (06) :674-678
[9]  
Miller J.H., 1992, SHORT COURSE BACTERI, V1
[10]   Nucleotide sequence and characterization of a novel cefotaxime-hydrolyzing β-lactamase (CTX-M-10) isolated in Spain [J].
Oliver, A ;
Pérez-Díaz, JC ;
Coque, TM ;
Baquero, F ;
Cantón, R .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (02) :616-620