Circadian modulation of calcium levels in cells in the suprachiasmatic nucleus

被引:131
作者
Colwell, CS [1 ]
机构
[1] Univ Calif Los Angeles, Dept Psychiat & Biobehav Sci, Mental Retardat Res Ctr, Los Angeles, CA 90024 USA
关键词
calcium oscillations; circadian rhythms; fura2; Rattus rattus; SCN;
D O I
10.1046/j.1460-9568.2000.00939.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
There is reason to believe that resting free calcium concentration [Ca2+](i) in neurons in the suprachiasmatic nucleus (SCN) may vary with the circadian cycle. In order to start to examine this hypothesis, optical techniques were utilized to estimate resting Ca2+ levels in SCN cells in a rat brain slice preparation. [Ca2+](i) measured from the soma was significantly higher in the day than in the night. Animals from a reversed light-dark cycle were used to confirm that the phase of the rhythm was determined by the prior light-dark cycle. The rhythm in Ca2+ levels continued to be expressed in tissue collected from animals maintained in constant darkness, thus confirming the endogenous nature of this variation. Interestingly, the rhythm in Ca2+ levels was not observed when animals were housed in constant light. Finally, the rhythm in Ca2+ levels was prevented when slices were exposed to tetrodotoxin (TTX), a blocker of voltage-sensitive sodium channels. Similar results were obtained with the voltage-sensitive Ca2+ channel blocker methoxyverapamil. These observations suggest a critical role for membrane events in driving the observed rhythm in Ca2+. Conceptually, this rhythm can be thought of as an output of the circadian oscillator. Because [Ca2+](i) is known to play a critical role in many cellular processes, the presence of this rhythm is likely to have many implications for the cell biology of SCN neurons.
引用
收藏
页码:571 / 576
页数:6
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