Localization of swine PRE-1 homologues in 13 loci of Phacochoerus aethiopicus and Tayassu tajacu genomes, and their sequence divergence

被引:10
作者
Sulandari, S
Muladno
Harumi, T
Yanai, S
Wada, Y
Yasue, H
机构
[1] NATL INST ANIM IND, DEPT ANIM BREEDING & GENET, ANIM GENOME RES GRP, TSUKUBA, IBARAKI 305, JAPAN
[2] SOC TECHNOINNOVAT AGR FORESTRY & FISHERIES, ANIM GENOME ANAL TEAM, TSUKUBA, IBARAKI 305, JAPAN
关键词
hippopotamus; peccary; PRE-1; swine SINE; warthog;
D O I
10.1111/j.1365-2052.1997.00108.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
In our previous study, PRE-1 (a swine short interspersed nuclear element, SINE) was found to be present in the genomes of animal species related to swine (Sus scrofa) i.e. warthog (Phacochoerus aethiopicus) and collared peccary (Tayassu tajacu) at almost the same frequency as in Sus scrofa. In the present study, we investigated whether PRE-1 was present in hippopotamus (Hippopotamus amphibius), which is in the same order but in a different family to Sus scrofa. Hippopotamus amphibius was found to contain no PRE-1. Then, in order to study the localization of PRE-1 sequences at locus level and the sequence divergence of the PRE-1 of individual loci among Sus scrofa, Phacochoerus aethiopicus and Tayassu tajacu, primer sets, which can amplify PRE-1 sequences at 13 loci of swine genome with the polymerase chain reaction, were prepared to identify any corresponding sequences in Phacochoerus aethiopicus and Tayassu tajacu. Twelve and nine of the 13 primer sets identified fragments in Phacochoerus aethiopicus and Tayassu tajacu respectively. Ten of the 12 Phacochoerus aethiopicus fragments and two of the nine Tayassu tajacu fragments contained PRE-1 sequences. Based on the divergence between the corresponding PRE-1 sequences at individual loci and on the mutation rate of the pseudogenes (r=4.6x10(-9)), Phacochoerus aethiopicus and Tayassu tajacu are currently calculated to have been separated from Sus scrofa later than 1.4 million years before present (MYBP) and 16.8 MYBP, respectively.
引用
收藏
页码:210 / 215
页数:6
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