Mediator-modified electrodes for catalytic NADH oxidation: high rate constants at interesting overpotentials

被引：32

作者：

Munteanu, FD

Mano, N

Kuhn, A

Gorton, L

机构：

[1] Lund Univ, Dept Analyt Chem, SE-22100 Lund, Sweden

[2] Ecole Natl Super Chim & Phys Bordeaux, Lab Anal Chim Reconnaissance Mol, F-33607 Pessac, France

来源：

BIOELECTROCHEMISTRY | 2002年 / 56卷 / 1-2期

关键词：

NADH oxidation; fluorenone; mediator; carbon paste electrode; cyclic voltammetry; rate constant;

D O I：

10.1016/S1567-5394(02)00009-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbon paste electrodes were modified with a nitrofluorenone derivative, 2,4,7-trinitro-9-fluorenone, adsorbed on zirconium phosphate (ZP). After electrochemical reduction of the fluorenone derivative, it turns into a very efficient mediator for electrocatalytic NADH oxidation, with a formal potential of about +250 mV vs. Ag/AgCl. The electrochemistry and the electrocatalytic properties of the mediator were investigated with cyclic voltammetry and rotating disk electrode methodology. The second order rate constant with NADH was evaluated and found to be higher than 10(6) m(-1) s(-1), thus approaching true diffusion controlled currents for NADH oxidation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：67 / 72

页数：6

共 35 条

[1]

Bard A. J., 1980, ELECTROCHEMICAL METH

[2]

BARTLETT P N, 1991, Progress in Reaction Kinetics, V16, P55

[3] Electrochemically triggered reaction of a surface-confined reagent:: Mechanistic and EQCM characterization of redox-active self-assembling monolayers derived from 5,5′-dithiobis(2-nitrobenzoic acid) and related materials [J].