The generation and use of recombinant extracellular vesicles as biological reference material

被引：122

作者：

Geeurickx, Edward ^{[1
,2
]}

Tulkens, Joeri ^{[1
,2
]}

Dhondt, Bert ^{[1
,2
,3
]}

Jan Van Deun ^{[1
,2
]}

Lippens, Lien ^{[1
,2
,4
]}

Vergauwen, Glenn ^{[1
,2
,5
]}

Heyrmari, Elisa ^{[1
]}

De Sutter, Delphine ^{[6
,7
]}

Gevaert, Kris ^{[2
,6
,7
]}

Impens, Francis ^{[2
,6
,7
,8
]}

Miinalainen, Ilkka ^{[9
]}

Van Bockstal, Pieter-Jan ^{[10
]}

De Beer, Thomas ^{[10
]}

Wauben, Marca H. M. ^{[11
]}

Nolte-'t-Hoen, Esther N. M. ^{[11
]}

Bloch, Katarzyna ^{[12
]}

Swinnen, Johannes, V ^{[12
]}

van der Pol, Edwin ^{[13
,14
,15
]}

Nieuwland, Rienk ^{[13
,15
]}

Braems, Geert ^{[5
]}

Callewaert, Nico ^{[2
,6
,7
]}

Mestdagh, Pieter ^{[2
,6
,16
]}

Vandesompele, Jo ^{[2
,6
,16
]}

Denys, Hannelore ^{[2
,4
]}

Eyckerman, Sven ^{[2
,6
,7
]}

De Wever, Olivier ^{[1
,2
]}

Hendrix, An ^{[1
,2
]}

机构：

[1] Univ Ghent, Dept Human Struct & Repair, Lab Expt Canc Res, B-9000 Ghent, Belgium

[2] Canc Res Inst Ghent, B-9000 Ghent, Belgium

[3] Ghent Univ Hosp, Dept Urol, B-9000 Ghent, Belgium

[4] Ghent Univ Hosp, Dept Med Oncol, B-9000 Ghent, Belgium

[5] Ghent Univ Hosp, Dept Gynaecol, B-9000 Ghent, Belgium

[6] Univ Ghent, Dept Biomol Med, B-9000 Ghent, Belgium

[7] VIB Ctr Med Biotechnol, B-9000 Ghent, Belgium

[8] VIB Prote Core, B-9000 Ghent, Belgium

[9] Univ Oulu, Bioctr Oulu, SF-90220 Oulu, Finland

[10] Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut Anal, Lab Pharmaceut Proc Analyt Technol, B-9000 Ghent, Belgium

[11] Univ Utrecht, Fac Vet Med, Dept Biochem & Cell Biol, NL-3584 CM Utrecht, Netherlands

[12] Univ Leuven, Dept Oncol, Lab Lipid Metab & Canc, B-3000 Leuven, Belgium

[13] Univ Amsterdam, Med Ctr, Lab Expt Clin Chem, NL-1105 AZ Amsterdam, Netherlands

[14] Univ Amsterdam, Med Ctr, Biomed Engn & Phys, NL-1105 AZ Amsterdam, Netherlands

[15] Univ Amsterdam, Med Ctr, Vesicle Observat Ctr, NL-1105 AZ Amsterdam, Netherlands

[16] Univ Ghent, Ctr Med Genet, B-9000 Ghent, Belgium

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

芬兰科学院; 欧盟地平线“2020”; 欧洲研究理事会;

关键词：

EXOSOMES; DOMAINS; CELLS;

D O I：

10.1038/s41467-019-11182-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Recent years have seen an increase of extracellular vesicle (EV) research geared towards biological understanding, diagnostics and therapy. However, EV data interpretation remains challenging owing to complexity of biofluids and technical variation introduced during sample preparation and analysis. To understand and mitigate these limitations, we generated trackable recombinant EV (rEV) as a biological reference material. Employing complementary characterization methods, we demonstrate that rEV are stable and bear physical and biochemical traits characteristic of sample EV. Furthermore, rEV can be quantified using fluorescence-, RNA- and protein-based technologies available in routine laboratories. Spiking rEV in biofluids allows recovery efficiencies of commonly implemented EV separation methods to be identified, intra-method and inter-user variability induced by sample handling to be defined, and to normalize and improve sensitivity of EV enumerations. We anticipate that rEV will aid EV-based sample preparation and analysis, data normalization, method development and instrument calibration in various research and biomedical applications.

引用

页数：12

共 41 条

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