Disruption of a sigma factor gene, sigF, affects an intermediate stage of spore pigment production in Streptomyces aureofaciens

被引：15

作者：

Rezuchova, B ^{[1
]}

Barak, I ^{[1
]}

Kormanec, J ^{[1
]}

机构：

[1] SLOVAK ACAD SCI,INST MOL BIOL,BRATISLAVA 84251,SLOVAKIA

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 153卷 / 02期

关键词：

sigma factor; differentiation; spore maturation; pigment production; gene disruption; Streptomyces;

D O I：

10.1016/S0378-1097(97)00276-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Streptomyces aureofaciens sigF gene encodes a sigma factor. By integrative transformation, via double cross-over, a stable null mutant of sigF gene was obtained. This mutation appeared to have no obvious effect on vegetative growth, but affected the late stage of spore maturation. Microscopic examination showed that spores were deformed, and spore wall was thinner, compared with the wild-type spores. The spore pigment of sigF mutant was green, compared to wild-type grey-pink spore pigmentation. The plasmid-born wild-type sigF gene complemented the mutation after transformation of the mutant strain.

引用

页码：371 / 377

页数：7

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