Preferential uptake of small-aggregate fraction of pulmonary surfactant in vitro

被引:14
作者
Horowitz, AD
Kurak, K
Moussavian, B
Whitsett, JA
Wert, SE
Hull, WM
McNanie, J
Ikegami, M
机构
[1] CHILDRENS HOSP, MED CTR, CHILDRENS HOSP RES FDN, DIV PULM BIOL, CINCINNATI, OH 45229 USA
[2] UNIV CALIF LOS ANGELES, LOS ANGELES CTY HARBOR MED CTR, SCH MED, DEPT PEDIAT, TORRANCE, CA 90509 USA
关键词
surfactant reuptake;
D O I
10.1152/ajplung.1997.273.2.L468
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Preferential uptake of small-aggregate fraction of pulmonary surfactant in vitro. Am. J. Physiol. 273 (Lung Cell. Mol. Physiol. 17): L468-L477, 1997.-Homeostasis of pulmonary surfactant requires metabolic clearance of surfactant forms with decreased surface activity. Rabbit pulmonary surfactant was labeled in vivo with rhodamine-labeled dipalmitoylphosphatidylethanolamine (R-DPPE), isolated, and fractionated into large- and small-aggregate subfractions by differential centrifugation. Endocytosis of large (LA)- and small (SA)-aggregate surfactant by a mouse lung epithelial cell line (MLE-12) was evaluated in vitro by epifluorescence microscopy. More SA than LA surfactant was taken up by MLE-12 cells. Endocytosis of SA and LA surfactant was inhibited by preincubation of the subfractions with surfactant protein A and 3.3 mM Ca2+. The difference in uptake between SA and LA surfactant was lost for reconstituted organic extracts of the subfractions. Much of the difference in uptake of SA and LA surfactant may be attributed to the greater concentration of surfactant protein A in LA surfactant.
引用
收藏
页码:L468 / L477
页数:10
相关论文
共 30 条
[1]   REVERSIBLE AND IRREVERSIBLE INACTIVATION OF PREFORMED PULMONARY SURFACTANT SURFACE-FILMS BY CHANGES IN SUBPHASE CONSTITUENTS [J].
AMIRKHANIAN, JD ;
TAEUSCH, HW .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1165 (03) :321-326
[2]   SP-A, SP-B, AND SP-C IN SURFACTANT SUBTYPES AROUND BIRTH - REEXAMINATION OF ALVEOLAR LIFE-CYCLE OF SURFACTANT [J].
BARITUSSIO, A ;
ALBERTI, A ;
QUAGLINO, D ;
PETTENAZZO, A ;
DALZOPPO, D ;
SARTORI, L ;
PASQUALIRONCHETTI, I .
AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (04) :L436-L447
[3]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[4]   BINDING, UPTAKE, AND LOCALIZATION OF SURFACTANT PROTEIN-B IN ISOLATED RAT ALVEOLAR TYPE-II CELLS [J].
BRESLIN, JS ;
WEAVER, TE .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (06) :L699-L707
[5]   UPTAKE OF LIPOSOMAL PHOSPHATIDYLCHOLINE BY GRANULAR PNEUMOCYTES IN PRIMARY CULTURE [J].
CHANDER, A ;
CLAYPOOL, WD ;
STRAUSS, JF ;
FISHER, AB .
AMERICAN JOURNAL OF PHYSIOLOGY, 1983, 245 (05) :C397-C404
[6]   LYSOPHOSPHATIDYLCHOLINE SENSITIZES LIPID EXTRACTS OF PULMONARY SURFACTANT TO INHIBITION BY SERUM-PROTEINS [J].
COCKSHUTT, AM ;
POSSMAYER, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1086 (01) :63-71
[7]   RAPID SENSITIVE METHOD FOR DETERMINING PHOSPHOLIPID PHOSPHORUS INVOLVING DIGESTION WITH MAGNESIUM-NITRATE [J].
DUCKCHONG, CG .
LIPIDS, 1979, 14 (05) :492-497
[8]   SURFACTANT CHEMICAL-COMPOSITION AND BIOPHYSICAL ACTIVITY IN ACUTE RESPIRATORY-DISTRESS SYNDROME [J].
GREGORY, TJ ;
LONGMORE, WJ ;
MOXLEY, MA ;
WHITSETT, JA ;
REED, CR ;
FOWLER, AA ;
HUDSON, LD ;
MAUNDER, RJ ;
CRIM, C ;
HYERS, TM .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (06) :1976-1981
[9]   SURFACTANT SUBTYPES OF MICE - METABOLIC RELATIONSHIPS AND CONVERSION INVITRO [J].
GROSS, NJ ;
NARINE, KR .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 67 (01) :414-421
[10]   SURFACTANT SUBTYPES IN MICE - CHARACTERIZATION AND QUANTITATION [J].
GROSS, NJ ;
NARINE, KR .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (01) :342-349