A new Rubisco-like protein coexists with a photosynthetic Rubisco in the planktonic cyanobacteria Microcystis

被引:23
作者
Carre-Mlouka, Alyssa
Mejean, Annick
Quillardet, Philippe
Ashida, Hiroki
Saito, Yohtaro
Yokota, Akiho
Callebaut, Isabelle
Sekowska, Agnieszka
Dittmann, Elke
Bouchier, Christiane
de Marsac, Nicole Tandeau
机构
[1] Inst Pasteur, Dept Microbiol, Unite Cyanobacteries, CNRS,URA 2172, F-75724 Paris 15, France
[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Nara 6300101, Japan
[3] Univ Paris 06, Dept Biol Structurale, Inst Mineral & Phys Milieux Condense, CNRS,UMR 7590, F-75252 Paris 05, France
[4] Univ Paris 07, Dept Biol Structurale, Inst Mineral & Phys Milieux Condense, CNRS,UMR 7590, F-75252 Paris 05, France
[5] HKU Pasteur Res Ctr, Hong Kong, Hong Kong, Peoples R China
[6] Humboldt Univ, Inst Biol, D-10115 Berlin, Germany
关键词
D O I
10.1074/jbc.M602973200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two genes encoding proteins related to large subunits of Rubisco were identified in the genome of the planktonic cyanobacterium Microcystis aeruginosa PCC 7806 that forms water blooms worldwide. The rbcL(I) gene belongs to the form I subfamily typically encountered in cyanobacteria, green algae, and land plants. The second and newly discovered gene is of the form IV subfamily and widespread in the Microcystis genus. In M. aeruginosa PCC 7806 cells, the expression of both rbcL(I) and rbcL(IV) is sulfur-dependent. The purified recombinant RbcL(IV) overexpressed in Escherichia coli cells did not display CO2 fixation activity but catalyzed enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate, and the rbcL(IV) gene rescued a Bacillus subtilis MtnW-deficient mutant. Therefore, the Microcystis RbcL(IV) protein functions both in vitro and in vivo and might be involved in a methionine salvage pathway. Despite variations in the amino acid sequences, RbcL(IV) shares structural similarities with all members of the Rubisco superfamily. Invariant amino acids within the catalytic site may thus represent the minimal set for enolization, whereas variations, especially located in loop 6, may account for the limitation of the catalytic reaction to enolization. Even at low protein concentrations in vitro, the recombinant RbcL(IV) assembles spontaneously into dimers, the minimal unit required for Rubisco forms I-III activity. The discovery of the coexistence of RbcL(I) and RbcL(IV) in cyanobacteria, the ancestors of chloroplasts, enlightens episodes of the chaotic evolutionary history of the Rubiscos, a protein family of major importance for life on Earth.
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收藏
页码:24462 / 24471
页数:10
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