Diboronic acids as fluorescent probes for cells expressing sialyl Lewis X

被引：112

作者：

Yang, WQ

Gao, SH

Gao, XM

Karnati, VVR

Ni, WJ

Wang, BH

Hooks, WB

Carson, J

Weston, B

机构：

[1] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

[2] Univ N Carolina, Dept Pediat, Chapel Hill, NC 27599 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 16期

关键词：

D O I：

10.1016/S0960-894X(02)00339-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A series of fluorescent diboronic acids was synthesized in nine steps as potential sensors for sialyl Lewis X (sLex). The fluorescent binding studies of these sensors with sLex were carried out in a mixed aqueous solution. Compound 7e was found to show the strongest fluorescence enhancement upon binding with sLex. Using cell cultures, 7e was shown to label sLex-expressing HEPG2 cells at 1 muM, while non-sLex-expressing cells were not labeled. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：2175 / 2177

页数：3

共 26 条

[1]

Adhikiri Devi P., 1999, Tetrahedron Letters, V40, P7893, DOI 10.1016/S0040-4039(99)01646-9

[2]

Dennis JW, 1992, CELL SURFACE CARBOHY, P161

[3] A new glucose-selective fluorescent bisboronic acid.: First report of strong α-furanose complexation in aqueous solution at physiological pH [J].