Subfertility and defective folliculogenesis in female mice lacking androgen receptor

被引:228
作者
Hu, YC
Wang, PH
Yeh, SY
Wang, RS
Xie, C
Xu, QQ
Zhou, XC
Chao, HT
Tsai, MY
Chang, CS [1 ]
机构
[1] Univ Rochester, Dept Pathol, George Whipple Lab Canc Res, Rochester, NY 14642 USA
[2] Univ Rochester, Dept Urol, George Whipple Lab Canc Res, Rochester, NY 14642 USA
[3] Taipei Vet Gen Hosp, Dept Obstet & Gynecol, Taipei 112, Taiwan
[4] Taipei Med Univ, Dept Obstet & Gynecol, Taipei 110, Taiwan
[5] Chang Gung Univ, Inst Reprod Med, Kaohsiung 833, Taiwan
关键词
D O I
10.1073/pnas.0404372101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The roles of the androgen receptor (AR) in female fertility and ovarian function remain largely unknown. Here we report on the generation of female mice lacking AIR (AR(-/-)) and the resulting influences on the reproductive system. Female AR(-/-) mice appear normal but show longer estrous cycles and reduced fertility. The ovaries from sexually mature AR(-/-) females exhibited a marked reduction in the number of corpora lutea. After superovulation treatment, the AR(-/-) ovaries produced fewer oocytes and also showed fewer corpora lutea. During the periovulatory period, an intensive granulosa apoptosis event occurs in the AR(-/-) preovulatory follicles, concurrent with the down-regulation of p21 and progesterone receptor expression. Furthermore, the defective conformation of the cumulus cell-oocyte complex from the AR(-/-) females implies a lower fertilization capability of the AR(-/-) oocytes. In addition to insufficient progesterone production, the diminished endometrial growth in uteri in response to exogenous gonadotropins indicates that AR(-/-) females exhibit a luteal phase defect. Taken together, these data provide in vivo evidence showing that AIR plays an important role in female reproduction.
引用
收藏
页码:11209 / 11214
页数:6
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